نتایج جستجو برای: machine sequencing

تعداد نتایج: 388534  

Journal: :Clinical chemistry 2013
Yuan Yuan Fuman Jiang Sang Hua Bole Du Yibin Hao Lili Ye Jiucheng Liu Kaiyan Feng Xinjie Huang Xin Yi Wei Wang Ling Yang Feng Mu Caixia Liu Yu Liang

BACKGROUND Noninvasive prenatal detection of common fetal aneuploidies with cell-free DNA from maternal plasma has been achieved with high-throughput next-generation sequencing platforms. Turnaround times for previously tested platforms are still unsatisfactory for clinical applications, however, because of the time spent on sequencing. The development of semiconductor sequencing technology has...

Journal: :Bioinformatics 2010
Anthony Mathelier Alessandra Carbone

MOTIVATION MicroRNAs (miRNAs) are a class of endogenes derived from a precursor (pre-miRNA) and involved in post-transcriptional regulation. Experimental identification of novel miRNAs is difficult because they are often transcribed under specific conditions and cell types. Several computational methods were developed to detect new miRNAs starting from known ones or from deep sequencing data, a...

Journal: :Nucleic Acids Research 2006
Patrick Ng Jack J.S. Tan Hong Sain Ooi Yen Ling Lee Kuo Ping Chiu Melissa J. Fullwood Kandhadayar G. Srinivasan Clotilde Perbost Lei Du Wing-Kin Sung Chia-Lin Wei Yijun Ruan

The paired-end ditagging (PET) technique has been shown to be efficient and accurate for large-scale transcriptome and genome analysis. However, as with other DNA tag-based sequencing strategies, it is constrained by the current efficiency of Sanger technology. A recently developed multiplex sequencing method (454-sequencing) using picolitre-scale reactions has achieved a remarkable advance in ...

2015
Wei Wu Chao-Xia Lu Yi-Ning Wang Fang Liu Wei Chen Yong-Tai Liu Ye-Chen Han Jian Cao Shu-Yang Zhang Xue Zhang

BACKGROUND MYBPC3 dysfunctions have been proven to induce dilated cardiomyopathy, hypertrophic cardiomyopathy, and/or left ventricular noncompaction; however, the genotype-phenotype correlation between MYBPC3 and restrictive cardiomyopathy (RCM) has not been established. The newly developed next-generation sequencing method is capable of broad genomic DNA sequencing with high throughput and can...

2010
Andrey Kislyuk Lee S. Katz Sonia Agrawal Matthew S. Hagen Andrew B. Conley Pushkala Jayaraman Viswateja Nelakuditi Jay C. Humphrey Scott A. Sammons Dhwani Govil Raydel D. Mair Kathleen M. Tatti Maria L. Tondella Brian H. Harcourt Leonard W. Mayer I. King Jordan

MOTIVATION New sequencing technologies have accelerated research on prokaryotic genomes and have made genome sequencing operations outside major genome sequencing centers routine. However, no off-the-shelf solution exists for the combined assembly, gene prediction, genome annotation and data presentation necessary to interpret sequencing data. The resulting requirement to invest significant res...

Journal: :Genome research 2014
Simone Picelli Asa K Björklund Björn Reinius Sven Sagasser Gösta Winberg Rickard Sandberg

Massively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel ...

2010
Sanjoy K Paul Abdullahil Azeem

This paper addresses the Hybrid Flow Shop (HFS) scheduling problems to minimize the total work-in-process inventory. Job scheduling problems are one of the oldest and real world combinational optimization problems. It is multi objective and complex in nature. There exist some criteria that must be taken into consideration when evaluating the quality of the proposed schedule. Consideration of jo...

Journal: :Computers & OR 2005
Qian Wang Rajan Batta Robert J. Szczerba

This paper considers the problem of sequencing the processing of incoming mail in a Processing and Distribution Center (P&DC) to match a given outbound truck delivery schedule. Mail arrives at a P&DC from local Associate Offices (AO) or remote P&DCs following an incoming schedule. The mail from an origin has different proportions to different destinations. For each destination, there are trucks...

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