introduction despite the fact that iran is one of the major chickpea (cicer arietinum l.) center of origins, limited information is available regarding chickpea genetic variation and diversity. genetic diversity information is crucial for the choice of proper parents to establish new breeding programs. chickpea germplasm is poor, suggesting the need for gaining enough knowledge of genetic diver...

The present work aims to molecularly characterize some popular Saudi fish species. Seven popular Saudi fish species namely, Morgan (Nemipteru sjaponicus), Mousa (Solea solea), Hamor (Greasy grouper), Shour (Lethrinus lentjan), Dennis (Caranxsex fasciatus), Harid (Scarus arabicus) and Black surgeon (Acanthurus gahhm) were characterized using six RAPD and three ISSR as well as Rep-PCR markers. Th...

DNA-based techniques have great potential for detecting genetic variability between genotypes. However, each approach generates different molecular markers, so the kind and amount of polymorphism detected and the cost and time required vary among them. In general, a good method for DNA fingerprinting to detect genetic polymorphism must generate a complex pattern of bands per experiment, the res...

Sex-specific DNA markers are useful for studying sex-determination mechanisms and establishment of monosex populations. Three widely spaced geographical populations (Liangzi, Poyang and Yuni Lakes in China) of blunt snout bream (Megalobrama amblycephala) were screened with AFLPs to search for sex-linked markers. Female and male pools (10 individuals in each pool) from each population were ...

Like bacteria, fungi play an important role in the soil ecosystem. As only a small fraction of the fungi present in soil can be cultured, conventional microbiological techniques yield only limited information on the composition and dynamics of fungal communities in soil. DNA-based methods do not depend on the culturability of microorganisms, and therefore they offer an attractive alternative fo...

The genetic relationships of A genomes of Triticum urartu (Au) and Triticum monococcum (Am) in polyploid wheats are explored and quantified by AFLP fingerprinting. Forty-one accessions of A-genome diploid wheats, 3 of AG-genome wheats, 19 of AB-genome wheats, 15 of ABD-genome wheats, and 1 of the D-genome donor Ae. tauschii have been analysed. Based on 7 AFLP primer combinations, 423 bands were...

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and inter simple sequence repeats (ISSRs) markers were performed to detect the genetic diversity among 6 new rice lines and 4 cultivars with different responses to drought tolerance and establish specific DNA markers associated with drought tolerance. Among 16 RAPD primers tested, only 5 produced bands polymorphic between lin...

data not shown). Figure 1 demonstrates the two alleles of exon 15 of hMLH1 obtained with and without the modifications described above. The modified technique resulted in unequivocal resolution in the complete absence of artifactual bands. Representative samples of hMLH1 exon 15 were subcloned (TA Cloning® Kit; Invitrogen, San Diego, CA, USA) and sequenced following manufacturer’s instructions ...

The molecular variability of ten genotypes of Celosia argentea seeds collected from National Institute of Horticultural Research (NIHORT) and National Centre for Genetic Resources and Biotechnology (NACGRAB) germplasms were evaluated using the Amplified Fragment Length Polymorphism (AFLP) marker. The polymorphism of C. argentea was detected within the population using primer mix of AFLP EcoRI +...