A lytic bacteriophage, which was previously isolated from sewage and which attaches to the K1 capsular antigen, has been used to prevent septicemia and a meningitis-like infection in chickens caused by a K1+ bacteremic strain of Escherichia coli. Protection was obtained even when administration of the phage was delayed until signs of disease appeared. The phage was able to multiply in the blood...

Despite knowledge about microbial responses to abiotic stress, few studies have investigated stress responses to antagonistic species, such as competitors, predators and pathogens. While it is often assumed that interacting populations of bacteria and phage will coevolve resistance and exploitation strategies, an alternative is that individual bacteria tolerate or evade phage predation through ...

P. aeruginosa (Kitasato strain) was used as host. Forty-three strains of P. aeruginosa from the culture collection of the Department of Bacteriology, School of Medicine, Kyushu University were used for determination of the lytic spectrum of the phage. A phage of P. aeruginosa with rod-shaped head was designated as K. The host cells were cultivated in nutrient broth with aeration for 3 hr. At th...

The antiviral effects of some pyrimidine derivatives were investigated using plaque reduction assay. Pyrimidine derivatives were dissolved in suitable vehicles and different concentrations of each compound were prepared. After several enriching stages of phage CP51, the phage numbers in phage lysates were determined. To ascertain whether pyrimidine derivatives have the ability to inhibit viral ...

three experiments were carried out for isolation of bacteriophage against growth reducing enterococcus faecalis and evaluation of its efficacy to control enterococci colonization in intestine of japanese quails. in experiment one, 60 enterococci spp were isolated from gastrointestinal contents of adult quails, which among them seven isolates were selected based on preliminary tests. in experime...

Many Staphylococcus aureus have lost a major genetic barrier against phage infection, termed clustered regularly interspaced palindromic repeats (CRISPR/cas). Hence, S. aureus strains frequently exchange genetic material via phage-mediated horizontal gene transfer events, but, in turn, are vulnerable in particular to lytic phages. Here, a novel strategy of S. aureus is described, which protects...

Complex and interacting selective pressures can produce bacterial communities with a range of phenotypes. One measure of bacterial success is the ability of cells or populations to proliferate while avoiding lytic phage infection. Resistance against bacteriophage infection can occur in the form of a metabolically expensive exopolysaccharide capsule. Here, we show that in Caulobacter crescentus,...

In these days that we are drifting into the post-antibiotic era, antibiotics called "last-resort" begun to be used more frequently. Colistin is one of last-resort act on Gram-negative bacteria. The aim study was investigate antibiotic re-sensitization effect lytic bacteriophages colistin resistant E. coli O157:H7 in-vitro. study, four isolates (encoded 25KA, 44RA, 120RA and 168KA) were included...

The prophage of coliphage N15 is not integrated into the chromosome, but exists as a linear plasmid molecule with covalently closed ends. The only phage gene required for replication of circular N15 miniplasmids is repA (gene 37). Here we show that RepA-driven replication of the N15-based circular and linear miniplasmids is independent of host DnaB helicase protein, but requires the host DnaG p...

This study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp. by using specific agents. Three bacteriophages and twelve strains of Salmonella spp. were tested. Each of the twelve strains was used separately to inoculate TSB toge...