MHC class I molecules expressed in a calreticulin-deficient cell line (K42) assembled with beta 2-microglobulin (beta2-m) normally, but their subsequent loading with optimal peptides was defective. Suboptimally loaded class I molecules were released into the secretory pathway. This occurred despite the ability of newly synthesized class I to interact with the transporter associated with antigen...

A helicon plasma sputtering system is used to deposit small amounts of platinum on microporous carbon support composed of Vulcan XC 72 carbon particles (known as gas diffusion layer GDL) to form Pt catalysed electrodes for proton exchange membrane fuel cells (PEMFC). Electrodes with low Pt loading are prepared, assembled in custom-made membrane electrode assemblies (MEAs) and tested for the hyd...

Background. Left ventricular mechanical efficiency is one of the most important measures of left ventricular pump performance. Several clinical studies, however, have shown that mechanical efficiency does not fall substantially as the heart fails. To clarify the insensitivity of mechanical efficiency to the change in pump performance, we analyzed human left ventricular mechanical efficiency, ap...

Hybrid halide perovskites represent one of the most promising solutions toward the fabrication of all solid nanostructured solar cells, with improved efficiency and long-term stability. This article aims at investigating the properties of CH3NH3PbI3 with controlled loading time in CH3NH3I solution via a two-step sequential deposition and correlating them with their photovoltaic performances. It...

We describe a simple, efficient, gentle and inexpensive technique for the introduction of normally impermeant macromolecules into the cytosol of living mammalian cells growing in suspension or attached to the culturing substratum. Loading is achieved by the production of transient, survivable plasma membrane disruptions as cells are passed back and forth through a standard syringe needle or sim...

a newly developed natural rubber deproteinization process produces deproteinized natural rubber (dpnr) wastewater as an intermediate product containing a high concentration of sodium dodecyl sulfate (sds) and rubber. in this study, a novel process to recover the residual rubber and energy as methane from dpnr wastewater was developed. as a pretreatment, sds and residual rubber in dpnr wastewate...

conclusions diclofenac-loaded nanoparticles based on properties of chitosan gelation could delay the release of drugs in the stomach and be further evaluated for the enteric delivery of diclofenac. objectives the aim of this study was to prepare and evaluate diclofenac-loaded chitosan nanoparticles based on the ionotropic gelation method. materials and methods chitosan (cs) nanoparticles contai...

introduction: there are various factors that affect manpower efficiency. identification of the most important and influential factors on efficiency is quite essential. analysis of factors affecting manpower efficiency from the viewpoint of clinical and non-clinical faculty members at guilan university of medical sciences in 2011. methods: this descriptive, analytical, cross-sectional study was ...

A novel cationic microbubble (MB) for improvement of the DNA loading capacity and the ultrasound-mediated gene delivery efficiency has been developed; it has been prepared with commercial lipids and a stearic acid modified polyethylenimine 600 (Stearic-PEI600) polymer synthesized via acylation reaction of branched PEI600 and stearic acid mediated by N, N'-carbonyldiimidazole (CDI). The MBs' con...