The Rab protein family belongs to a superfamily of ras-like GTP-binding proteins. Rab proteins regulate many steps of membrane trafficking. In this study, three Rab family members, Rab5B, Rab6A, and Rab7, designated LvRab5B, LvRab6A, and LvRab7, were cloned from Litopenaeus vannamei. The full-length cDNA sequences of LvRab5B, LvRab6A, and LvRab7 were 1383, 873, and 767 nucleotides in length and...

Objective. The present study aimed to investigate the presence of vibrios in the hepatopancreas of cultured shrimp. Materials and methods. Vibrios from the hepatopancreas of fifteen samples of five specimens each, of apparently healthy Pacific white shrimp (Litopenaeus vannamei) were isolated, identified and quantified. Results. The vibrio density ranged from 430 to 2,400 MPN g-1 (rs MPN cm-1=-...

Acute hepatopancreatic necrosis disease (AHPND) is a severe shrimp disease originally shown to be caused by virulent strains of Vibrio parahaemolyticus (VPAHPND). Rare cases of AHPND caused by Vibrio species other than V. parahaemolyticus were reported. We compared an AHPND-causing V. campbellii (VCAHPND) and a VPAHPND isolate from the same AHPND-affected pond. Both strains are positive for the...

The toxic effect of the dinoflagellate Prorocentrum lima on juvenile American whiteleg shrimp Litopenaeus vannamei and giant lion-paw scallop Nodipecten subnodosus was evaluated. Organisms were exposed to three densities (500, 2000, or 5000 cells mL(-1)), superoxide dismutase activity and soluble protein in the hepatopancreas and muscle were determined at 1, 6, 24 and 48 h after challenge. Shri...

A highly pure, chemically defined representative of a new class of antimicrobial peptide from the Atlantic white shrimp (Litopenaeus setiferus), penaeidin class 4 [Pen4-1 (penaeidin class 4 isoform 1)], was produced synthetically. Chemical synthesis was achieved by native ligation from two separate domains yielding a bioactive peptide that reflected the characteristics of native penaeidin. Synt...

Two experimental feeding trials were conducted during four weeks to evaluate the use of Ulva lactuca in shrimp culture: (1) for wastewater bioremediation, and (2) using different inclusion levels of U. lactuca meal in shrimp feed. In feeding trial 1, shrimp reared under seaweed U. lactuca water exchange in a re-circulation system (SWE) resulted in similar growth and feed utilization as shrimp r...

Pemberian pakan alami berupa mikroalga merupakan salah satu faktor penting dalam pembenihan udang vaname (Litopenaeus vannamei). Penelitian ini bertujuan untuk mengkaji pengaruh pemberian yang berbeda terhadap pertumbuhan dan perkembangan fase stadia larva vaname. dilakukan berdasarkan studi kasus pada dua panti benih terletak di Kabupaten Situbondo Jawa Timur. Panti Benih A menggunakan Skeleto...

Antimicrobial peptides are important innate immune defense, especially in those animals which lack adaptive immunity [1e8]. Due to their small size, amphipathic structure and cationic character they can rapidly diffuse to the point of infection [9], a mechanism that presumably makes it easier to circumvent microbial resistance against the peptides [10]. Besides providing an immediate and broad-...

Tingginya padat tebar dalam budidaya udang vaname (Litopenaeus vannamei) intensif mengakibatkan penumpukan bahan organik yang dapat menimbulkan penyakit dan kegagalan budidaya. Sehingga perlu penggunaan sistem manajemen kualitas air baik untuk menjaga parameter tetap optimal. Sistem semi bioflok merupakan berkembang di Indonesia dengan beberapa keuntungan salah satunya terjaganya karena aktivit...

Teknologi Zero Water Discharge merupakan salah satu teknologi yang dapat diterapkan pada budidaya udang vaname (Litopenaeus vannamei) menggunakan kolam terpal skala kecil. Kegiatan ini bertujuan untuk meningkatkan pengetahuan tentang zero water dischargepada kelompok tani Ombo Mandiri Kelurahan Wolulu Kecamatan Watubangga Kabupaten Kolaka. dilaksanakan tahun 2020 dalam bentuk penyuluhan, pelati...