The entrapment of anticancer drug ellipticine in the dipalmitoylphosphocholine (DPPC) liposome and its release by addition of three different bile salts, namely sodium deoxycholate, cholate and taurocholate, have been studied by steady state and time resolved fluorescence spectroscopy. We found that the release of the drug from a liposome depends on the degree of penetration of bile salts. Amon...

The interaction between single-stranded RNAs and liposomes was studied using UV, Fourier Transform Infrared spectroscopy (FTIR) and Circular Dichroism spectroscopy (CD). The effect of the surface characteristics of liposomes, which were composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and modified with cholesterol (Ch) or 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), on t...

Fusion of biological membranes is a fundamental process in many cellular functions, including the intracellular delivery of lipids, proteins, and metabolites by transport vesicles, endocytosis, exocytosis, cell division, cell-cell fusion, fertilization, and the invasion of cells by enveloped viruses. These complex fusion processes involve proteins that bring the fusing membranes into close prox...

A thorough understanding of interactions occurring at the interface between nanocarriers and biological systems is crucial to predict and interpret their biodistribution, targeting, and efficacy, and thus design more effective drug delivery systems. Upon intravenous injection, nanoparticles are coated by a protein corona (PC). This confers a new biological identity on the particles that largely...

cyproterone acetate (ca) has been loaded to liposome by solvent evaporation and thin film formation technique. the effects of some formulation variables such as temperature of organic solvent evaporation, rotary evaporator speed, volume of organic solvent, volume of balloon and temperature of hydrating buffer has been evaluated. the data showed that bigger balloon with higher surface area has b...

background and objectives:  the most common problems limiting the medical use of aminoglycosides have been the nephro- and oto-toxicities as well as the increasing bacterial resistance. encapsulation of drugs into liposomes enhances their efficacy while reducing their toxicities. the aim of this study was to evaluate the antimicrobial activity of free and liposomal amikacin. material and method...

Possible complete closure of hydrophilic drug solutions in liposomes with required dimensions is the aim of variety liposome techniques. The ease of separating medication-loaded liposomes from liposome suspension to achieve an appropriate drug concentration in the final preparation is also desired. This paper describes the use of liposome preparation method, called reverse-phase evaporation, wh...

Conjugation of protein to liposomes by two coupling protocols is shown to result in vesicle aggregation. The degree of aggregation is directly related to the levels of protein conjugated to the liposomes. In an attempt to develop a method of generating stable, homogeneously sized protein-conjugated vesicles, highly aggregated liposome-protein conjugates were extruded through filters of defined ...

A study has been made to determine if the cytotoxicity observed when cells in culture were exposed to liposome-entrapped cytotoxic drugs was liposome mediated or resulted from leakage of drug from the liposomes with subsequent uptake of free drugs by the cells. In preliminary experiments with the EMT6 cell line in monolayer culture, the cytotoxicity observed when the cells were exposed to a ran...