The performance of a commercial immunofluorescence assay (IFA commercial), an in-house in-house) and indirect enzyme-linked immunosorbent (ELISA) were evaluated in the detection antibodies anti-C. burnetii serum Q fever patients persons without disease. For study, seropositive seronegative samples for (n = 200) from bank Instituto Adolfo Lutz Brazil used. Commercial IFA was considered this stud...

the purpose of this survey was to evaluate natural occurrence and content of aflatoxin m1 (afm1) in dairy products marketed in urmia (iran). during september 2007, 40 samples of pasteurized milk, 40 samples of ultra high temperature treated (uht) milk, 40 samples of creamy cheese and 40 samples of iranian feta cheese were collected from different supermarkets in urmia city. afm1 contents were d...

An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid and simple differentiation of toxigenic from nontoxigenic strains of Pasteurella multocida. The sandwich ELISA is based on two different murine monoclonal antibodies with specificity for the P. multocida toxin. The ELISA, which is now used as a routine test in Denmark, has several advantages compared with previously descri...

A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

An enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibodies to Histophilus somni exopolysaccharide (EPS), which is created during biofilm formation. When an index value of 0.268 was used, the sensitivity of the assay for infected calves was 90.5% at 3 weeks postinfection, but the number of positive animals increased by week 4. The specificity of the assay for healthy ...

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...