The PGA enzyme belongs to the family of N-terminal nucleophile (Ntn) hydrolases. The enzyme Penicillin G Acylase (PGA) mainly produced from Penicillium chrysogenum, E.coli. The synthesis of PGA enzyme is depends on the physico-chemical parameters like carbon source, pH, temperature and media were optimized for higher production of enzyme. There are different methods of purification of PGA enzym...

Until now, only recombinant forms of dipeptidyl peptidase (DPP) 8 and 9 have been characterized. We purified non DPPII-non DPPIV enzymes from a natural source. A first DPP8/9-like enzyme was enriched 1160-fold from bovine testes and identified as 'DPP9-like enzyme' by using an anti-DPP9 antibody. A second 576-fold enriched preparation ('DPP enriched peak 3') also showed DPP8/9-like activity. SD...

The protease enzyme produced by Lactic Acid Bacteria (LAB) with characteristics similar to rennin is called Rennin Like Protease (RLP). purpose of the study was obtain information on biochemical RLP Enterococcus faecium 1.15 as a basis for utilization enzyme. first stage observation growth curves and potential isolates produce RLP, characterization. (RLP) which has been purified using Sephadex ...

Four new ACTG-toxins like meroterpenoids tricycloalternarenes O-R were discovered from a marine-derived fungus Alternaria alternata JJY-32 by the overexpression of LaeA-like global transcriptional regulator and MpaB-like biosynthetic tailoring enzyme. Among those structures, tricycloalternarene O possesses non-canonical cyclohexanone-pyran fused furan ring structure R contains truncated isopren...

Aortic aneurysm is a chronic degenerative condition associated with atherosclerosis. Recent studies have revealed that angiotensin (Ang) II plays important roles in atherosclerosis. In this study, to investigate the relationship between aortic aneurysm and Ang II, we measured the activities of the angiotensin (Ang) II-forming enzymes, angiotensin converting enzyme (ACE) and chymase-like enzyme,...

Xanthine phosphoribosyltransferase (XPRT) and hypoxanthine-guanine (HGPRT) are purine salvaging enzymes of Leishmania donovani with distinct 6-oxopurine specificities. LdXPRT phosphoribosylates xanthine, hypoxanthine, guanine, preference toward whereas LdHGPRT only hypoxanthine guanine. In our study, was used as a model to understand these base Mutating I209 V, the conserved residue found in HG...