We used ion-exchange column chromatography and electrophoresis on polyacrylamide gel to compare the acid phosphatase isoenzymes of prostate and leukocytes. The major isoenzyme of the prostate is band 2A; only a trace of band 2B was observed. However, the major isoenzymes of leukocytes are band 4 and band 2B, and only a small amount of band 2A was observed. The three isoenzymes isolated from leu...

The entry of leukocytes into tissues requires well-coordinated interactions between the immune cells and endothelial cells which form the inner lining of blood vessels. The molecular basis for recognition, capture, and adhesion of leukocytes to the endothelial apical surface is well studied. This review will focus on recent advances in our understanding of events following the firm interaction ...

Abstract Leukocyte recruitment and their mediated inflammatory responses are critical for cardiovascular diseases, including myocardial ischemia-reperfusion (I/R) injury. Blocking leukocyte in mouse knockouts (KO) of beta2 integrin (CD18) or blocking CD18 with antibodies multiple animals significantly reduced infarct size after I/R However, the cell-specific contribution to injury is unknown. I...

Gangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation, growth control, and signaling. However, the role that gangliosides play in the immune rejection response after xenotransplantation is not yet clearly understood. In this study, the regula...

This study evaluated the effects of dietary vitamin A and E on the in vitro capacity of blood mononuclear leukocytes from calves to produce nitric oxide. Calves fed milk replacer received 100 IU/d of vitamin E as RRR-alpha-tocopherol or RRR-alpha-tocopheryl acetate and 0, 1700, 34,000, or 68,000 IU of vitamin A as retinyl acetate. Leukocytes from calves produced greater amounts of nitric oxide ...

Chronic exposure to coalmine dust is associated with the accumulation of inflammatory leukocytes in the bronchoalveolar region of the lung and, in the long term, with fibrosis and emphysema of the lung parenchyma. Degradation of connective tissue by inflammatory leukocytes has been implicated in the parenchymal damage that precedes the development of fibrotic or emphysematous lesions in the lun...

A small proportion of patients with acute or chronic leukemia has an extraordinarily high blood leukocyte count. These high counts can result in a very high fractional volume of leukocytes (leukocrit), which is also a function of the mean leukocyto volume in different types of leukemia. Despite a high fractional volume of leukocytes, bulk viscosity of blood is usually not increased because a de...

Bone marrow cells and peripheral blood leukocytes from leukemic AKR/J mice synthesize sterols from acetate at a vastly greater rate than the respective cells from normal AKR/J mice, i.e., approximately 20and 100-fold. The controlling enzyme of sterol synthesis, 3-hydroxy-3-melhylglutaryl coenzyme A reducÃ-ase, is readily detectable in gradient-purified leukocytes from leukemic mice, whereas nor...

A B S T R A C T Human polymorphonuclear leukocytes (PMN) phagocytosing opsonized antigen-antibody complexes, produce dialyzable species of activated oxygen which are capable of partially suppressing the elastase-inhibiting capacity (EIC) of whole human serum or purified human a1-proteinase inhibitor. Serum EIC was partially protected by superoxide dismutase, catalase, or mannitol, suggesting th...

Leukocyte tracking invivo is a state of the techniques used for understanding the complex nature of leukocyte recruitment and the transmigration through endothelial cells. It known that the governing force acting on the individual cells are mainly hydrodynamic, the magnitude and nature of the force is not precisely known. This work was a novel segmentation algorithms based on fuzzy logic to tra...