In the present study, a 0.47 kb OMT gene construct from aspen, encoding for an enzyme O-methyltransferase (OMT, EC 2.1.1.6), in antisense orientation was used to down-regulate lignin biosynthesis in Leucaena leucocephala. The plants were transformed with Agrobacterium tumefaciens strain harboring the antisense gene, and the transformation was confirmed by PCR amplification of the npt II gene. T...

An in vivo trial was conducted in sheep to investigate the effect of three tropical tannin-rich plants (TRP) on methane emission, intake and digestibility. The TRP used were leaves of Glyricidia sepium, Leucaena leucocephala and Manihot esculenta that contained, respectively, 39, 75 and 92 g condensed tannins/kg DM. Methane was determined with the sulphur hexafluoride tracer technique. Eight ru...

Twenty four Osmanabadi weaned kids of same age and uniform confirmation was selected for experimental trails. The were grouped under weight average in treatment group with six (3male 3female) each group. control (T0) included 80% DCP through concentrate mixture + 20% green Sorghum sorghum Kadbi should be given ad-lib; T1 +20% fodder+ 30% from Sesbania grandiflora (Hadaga) foliage Leucaena leuco...