نتایج جستجو برای: leishman and giemsa stain

تعداد نتایج: 16828732  

Journal: :Journal of the Medical Association of Thailand = Chotmaihet thangphaet 2005
Siraya Jaijakul Wilai Saksirisampant Juraratt Prownebon Sutin Yenthakam Mathirut Mungthin Saovanee Leelayoova Surang Nuchprayoon

OBJECTIVES To detect P. jiroveci (previously named P. carinii) by PCR using FTA filter paper to extract the DNA, from noninvasive induced sputum samples of HIV/AIDS patients. MATERIAL AND METHOD Fifty two HIV/AIDS patients suspected of Pneumocystis jiroveci pneumonia (PJP) in King Chulalongkorn Memorial Hospital were recruited. Both cytological method and PCR with FTA filter paper technique w...

2017
Abedin Saghafipour Hassan Vatandoost Ali Reza Zahraei-Ramazani Mohammad Reza Yaghoobi-Ershadi Moharram Karami Jooshin Yavar Rassi Mohammad Reza Shirzadi Amir Ahmad Akhavan Ahmad Ali Hanafi-Bojd

Background Cutaneous leishmaniasis (CL) is one of the most important health problems in many areas of Iran. There are two forms of the disease in Iran, anthroponotic and zoonotic CL. This study conducted to assess the epidemiological situation of CL in an endemic area of Qom Province, central Iran from Apr to Nov 2015. Methods The sticky paper traps and aspirating tubes were used for collecti...

Journal: :Parasitology 2014
J Russell Stothard Betty Nabatte Jose C Sousa-Figueiredo Narcis B Kabatereine

Malaria microscopy in sub-Saharan Africa is often restricted by access to light microscopes. To address this gap, a novel portable inverted monocular microscope, the Newton Nm1, was designed and is now commercially available. Its diagnostic performance was assessed in a blinded-slide trial at ×1000 (oil) of Giemsa-stained thick blood films against a conventional microscope as undertaken by four...

Journal: :Journal of clinical microbiology 2010
Bernard Nkrumah Alex Agyekum Samuel E K Acquah Jürgen May Egbert Tannich Norbert Brattig Samuel Blay Nguah Heidrun von Thien Yaw Adu-Sarkodie Frank Huenger

Malaria remains the single most frequent cause of death in Africa, killing one child every 30 s, but treatment decisions are often made only on clinical diagnosis, as laboratory techniques to confirm the clinical suspicion are labor intensive and costly. In this study, we evaluated the recently developed Partec rapid malaria test (PM) for the detection of Plasmodium spp. in human blood from pat...

2010
Yaye Ramatoulaye Lawaly Anavaj Sakuntabhai Laurence Marrama Lassana Konate Waraphon Phimpraphi Cheikh Sokhna Adama Tall Fatoumata Diène Sarr Chayanon Peerapittayamongkol Chalisa Louicharoen Bradley S. Schneider Anaïs Levescot Arthur Talman Isabelle Casademont Didier Menard Jean-François Trape Christophe Rogier Jaranit Kaewkunwal Thanyachai Sura Issarang Nuchprayoon Frederic Ariey Laurence Baril Pratap Singhasivanon Odile Mercereau-Puijalon Rick Paul

BACKGROUND Studies on human genetic factors associated with malaria have hitherto concentrated on their role in susceptibility to and protection from disease. In contrast, virtually no attention has been paid to the role of human genetics in eliciting the production of parasite transmission stages, the gametocytes, and thus enhancing the spread of disease. METHODS AND FINDINGS We analysed fou...

Journal: :Acta cytologica 1995
C Armbruster L Pokieser A Hassl

OBJECTIVE To assess the sensitivity, specificity and accuracy of Diff-Quik, fungifluor stain, the direct immunofluorescence test (DIFT) and the polymerase chain reaction (PCR) in the diagnosis of Pneumocystis carinii pneumonia (PCP) in human immunodeficiency virus (HIV)-infected patients. STUDY DESIGN From December 1992 through November 1993, 112 bronchoalveolar lavage fluid (BALF) samples we...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2002
Yoshihito Niimura Takashi Gojobori

Giemsa staining has been used for identifying individual human chromosomes. Giemsa-dark and -light bands generally are thought to correspond to GC-poor and GC-rich regions; however, several experiments showed that the correspondence is quite poor. To elucidate the precise relationship between GC content and Giemsa banding patterns, we developed an "in silico chromosome staining" method for reco...

2007
Umar Isa Ibrahim Albert Wulari Mbaya Hayatu Mahmud Ali Mohammed

The prevalence of cryptosporidial infection among captive wild animals and birds in Sanda Kyarimi Park, Maiduguri, north-eastern Nigeria, was evaluated by faecal examination for oocysts using three different staining techniques: the modified Ziehl-Neelsen, Giemsa, and Safranin-methylene blue. A total of 66 captive wild animals and birds were examined based on age and sex differences. Fifteen (2...

Journal: :Journal of clinical pathology 1997
C Doglioni M Turrin E Macrì C Chiarelli B Germanà M Barbareschi

AIMS To verify whether the proposed new silver staining method compares favourably with other well established methods in the detection of Helicobacter pylori in gastric biopsies. METHODS One hundred and forty pairs of antral and fundic biopsies, routinely formalin fixed and paraffin wax embedded, from 70 consecutive unselected patients were stained with haematoxylin and eosin, modified Giems...

2016
Shahina Mumtaz Asif Hussain

Objective: to know and document the risk of factors for category leishmaniasis. Material and Methods: Cutaneous leishmaniasis is rapidly and easily diagnosed by direct examination of impression smear from lesion. Smear was stained by giemsa or Wright stain. In active lesion amastigote form of parasite are easily recognized intracellularly and extracellularly. Diagnosis of visceral leishmaniasis...

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