White flagging of alfalfa, Medicago sativa 'Ranger, found associated with Ditylenchus dipsaci in the Columbia River Basin was observed in northern Utah during 1971. This is a report on chloroplast changes, induced by D. dipsaci in alfalfa leaves, as observed with an electron microscope. Leaves from alfalfa plants infected with D. dipsaci were either devoid of normal pigmentation or displayed va...

A developmental validation study based on recommendations of the Scientific Working Group on DNA Analysis Methods (SWGDAM) was conducted on a multiplex system of 10 Cannabis sativa short tandem repeat loci. Amplification of the loci in four multiplex reactions was tested across DNA from dried root, stem, and leaf sources, and DNA from fresh, frozen, and dried leaf tissue with a template DNA ran...

The physiological mechanisms underlying leaf growth inhibition under salt stress are not fully understood. Apoplastic pH is considered to play an important role in cell wall loosening and tissue growth and was demonstrated to be altered by several growth-limiting environmental conditions. In this study we have evaluated the possibility that inhibition of maize (Zea mays) leaf elongation by sali...

The present study was conducted to evaluate the hepatoprotective effects of an aqueous leaf extract of Coccinia grandis on carbon tetrachloride (CCl4) induced liver damage in albino rats. Wistar albino rats weighing around 180-200g were used. Toxicity was induced by using 30% CCl4 suspended in olive oil (1.0 ml/kg body wt intraperitoneal) after every 72 hrs for 3doses. The aqueous leaf extract ...

In the dark, leaf tissue of crisphead lettuce (Lactuca sativa L.) metabolized (14)CO to (14)CO(2) and acid-stable products. Tissue incubated at 2.5 degrees C for 3.5 hours and 48 hours converted about 1% and 17%, respectively, of the applied (14)CO to (14)CO(2), and incorporated about 0.04% and 0.6% of the (14)C in acid-stable products. Examination of soluble acid-stable products from (14)CO an...

Mature maize leaf tissue (Zea mays L.) was immunolabeled using a pre-embedding protocol with specific antibodies for nitrate reductase and protein A-colloidal gold. Immunogold label was found exclusively in the cytoplasm of mesophyll cells; no reaction was detected in bundle sheath cells. Chloroplasts, which were sliced open during cryosectioning, had no labeling. Thus, it appears nitrate reduc...