Highly active cytochrome b6f complexes from spinach and the cyanobacterium Mastigocladus laminosus have been analyzed by liquid chromatography with electrospray ionization mass spectrometry (LCMS ). Both sizeexclusion and reverse-phase separations were used to separate protein subunits allowing measurement of their molecular masses to an accuracy exceeding 0.01% ( 3 Da at 30,000 Da). The produc...

The design, preparation, and properties of planar multilayer structures composed of various combinations of sequentially deposited polyelectrolyte (PE) chains and self-assembled layers of individual block copolymer aggregates (vesicles, micelles, or large compound micelles (LCMs)) are described. The aggregates contain negatively or positively charged corona chains while the PE multilayers conta...

Anabolic steroids are widely abused in animal sports to improve their performance. The present study identifies the possible metabolites of boldenone camel liver. A high-resolution accurate QE mass spectrometer was used identify parent and its metabolites. To investigate phase 1 biotransformation camel, sodium phosphate buffer NADPH were used. Chromatographic separation carried out on a Thermo ...

OBJECTIVE The early diagnosis of rheumatoid arthritis (RA) is desirable to install treatment to prevent disease progression and joint destruction. Autoantibodies and immunological markers pre-date the onset of symptoms by years albeit not all patients will present these factors, even at disease onset. Additional biomarkers would be of high value to improve early diagnosis and understanding of t...

BACKGROUND Albumin glycation and subsequent formation of advanced glycation end products (AGEs) correlate with diabetes and associated complications. METHODS Human Serum Albumin (HSA) was modified with D-glucose for a 40 day period under sterile conditions at 37°C. Modified samples along with native HSA (unmodified) were analyzed for structural modifications by UV and fluorescence, FTIR, Liqu...

The onslaught of pathogens (disease-causing microbes) like bacteria and mold is responsible for the majority cases food deterioration. various techniques preservation that have been developed are all intended to lessen or completely get rid harmful ingredients. term "food preservation" refers methods preserving food. Food preservative improves human health by eradicating restricting growth orga...

Forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea, FCF) is a plant growth regulator, being extensively used for increasing kiwifruit size. The toxicological properties of its may persist in their transformation products (TPs) or even higher toxicity than FCF. TPs elucidation of FCF in postharvest kiwifruit (Actinidia chinensis, Chinese gooseberry) by the liquid chromatography ionization hybr...

OBJECTIVES The aim of this study was to evaluate the association of sex hormones with anthropometry in a large population-based cohort, with liquid chromatography-mass spectrometry (LCMS)-based sex hormone measurements and imaging markers. STUDY DESIGN/MAIN OUTCOME MEASURES Cross-sectional data from 957 men and women from the population-based Study of Health in Pomerania (SHIP) were used. Ass...