Multi-fluorescence recovery after photobleaching is a microscopy technique used to measure the diffusion coefficient (or analogous transport parameters) of macromolecules, and can be applied to both in vitro and in vivo biological systems. Multi-fluorescence recovery after photobleaching is performed by photobleaching a region of interest within a fluorescent sample using an intense laser flash...

Scanning electron microscopy is a useful imaging approach for the visualization of bacterial biofilms in their natural environments including their medical and dental habitats, because it allows for the exploration of large surfaces with excellent resolution of topographic features. Most biofilms in nature, however, are embedded in a thick layer of extracellular matrix that prevents a clear ide...

The LiF:F2+** laser has been shown' to provide high power (hundreds ofmJ), efficient (tens of%), room temperature stable laser operation tunable from 800 nm to nearly 1210 nm. However, no reasonable explanations were provided for such an ultrabroadband tuning capability using this active medium. This work describes the mechanism ofthe ullrabroadband lasing from LiF:F2+** und Alexandrite 1asa pu...

PURPOSE The purposes of this study were to: (1) evaluate the ability of a laser fluorescence (LF) unit to detect simulated caries under pit and fissure sealants; (2) determine the effect of an opacifying agent in sealants on LF values; and (3) determine interexaminer reproducibility values of the unit in a highly controlled, laboratory setting. Sealant characteristics specifically considered we...

Stimuli-Responsive Fluorescence Switching In article number 2301228, Bing Yang, Haichao Liu, and co-workers, report a noncovalent fluorescence switching achieved by utilizing the competition of H-bond π–π interaction. The obvious transition between blue monomer emission yellow dimer can be induced laser radiation used for rewritable optical recording time-dependent anti-counterfeiting.

Femtosecond-laser-written integrated waveguides enable multi-wavelength fluorescence sensing of flowing biomolecules in an optofluidic chip. Fluorescence from differently labeled biomolecules with distinct absorption wavelengths, encoded by uniquely modulating each excitation beam, is detected by a color-blind photodetector, and the origin of each signal is unraveled by Fourier analysis. Keywor...

Two-photon fluorescence microscopy has become increasingly popular in biomedical research as it allows high-resolution imaging of thick biological specimen with superior contrast and penetration than confocal microscopy. However, two-photon microscopy still faces two fundamental limitations: 1) image-contrast deterioration with imaging depth due to out-of-focus background and 2) diffraction-lim...

In article number 2100315, Hong Xu and co-workers develop a huge barcode library containing 300 kinds of encoded microbeads through novel structure-fluorescence combinational strategy. These barcodes similar to celestial bodies in the starry sky could be excited by single laser achieve decoding three elements, including structure two fluorescence information. The platform exhibits great promisi...

Over the past years, classical electrophysiological approaches to elucidate the functioning of nerve cells have been complemented by functional optical methods, in particular fluorescence imaging. This review illustrates how optical methods have proved helpful in the analysis of the neuronal principles underlying visual motion processing in the fly, a model system which allows physiological inv...

BACKGROUND With regard to the importance of life skills, the present study deals with the effect of life skills training on general health of the students. MATERIALS AND METHODS This was a one-group, pre-test, post-test, quasi-experimental research. Forty students were selected through purposive sampling method. The data collection tool was Goldberg General Health Questionnaire. RESULTS Pai...