The far-red fluorescent protein mKate (lambda(ex), 588 nm; lambda(em), 635 nm; chromophore-forming triad Met(63)-Tyr(64)-Gly(65)), originating from wild-type red fluorescent progenitor eqFP578 (sea anemone Entacmaea quadricolor), is monomeric and characterized by the pronounced pH dependence of fluorescence, relatively high brightness, and high photostability. The protein has been crystallized ...

The optical spectrum of reduced myeloperoxidase (EC 1.11.1.7) displays an unusual red shift of the Soret band which is at 472 nm and the alpha-band which is at 636 nm. The spectral properties of myeloperoxidase can be modified by means of acid treatment. Upon short exposure to acid (pH 1.7) the red-shifted optical absorption spectrum of the reduced enzyme (lambda max at 472 nm) was blue-shifted...

We investigate the $f$-mode oscillation of dark matter admixed hyperon star within relativistic Cowling approximation. The macroscopic properties are calculated with mean-field equation states by assuming that particles inside star. frequencies (only for $l=2$) four different neutron states. also check effects hyperons/dark and hyperons on oscillations varying astrophysical quantities such as m...

Leaf chlorophyll content provides valuable information about physiological status of plants. Reflectance measurement makes it possible to quickly and non-destructively assess, in situ, the chlorophyll content in leaves. Our objective was to investigate the spectral behavior of the relationship between reflectance and chlorophyll content and to develop a technique for non-destructive chlorophyll...

in this paper, we investigate the existence of positive solutions for the ellipticequation $delta^{2},u+c(x)u = lambda f(u)$ on a bounded smooth domain $omega$ of $r^{n}$, $ngeq2$, with navier boundary conditions. we show that there exists an extremal parameter$lambda^{ast}>0$ such that for $lambda< lambda^{ast}$, the above problem has a regular solution butfor $lambda> lambda^{ast}$, the probl...

The present study offers a new method based on CdSe quantum dots (QDs) for simultaneous separation and determination of trace levels of heparin (Hep) in human serum samples. In this technique, CdSe QDs perform two different functions in Hep analysis process. Mercaptoacetic acid-capped red CdSe QDs (&lambda;ex=690 nm) are conjugated to Hep and the Hep-QD conjugation is then used as an extraction...

The ability to control the level of gene expression is a major quest in biology. A widely used approach employs deletion of a nonessential gene of interest (knockout), or multi-step recombineering to move a gene of interest under a repressible promoter (knockdown). However, these genetic methods are laborious, and limited for quantitative study. Here, we report a tunable CRISPR-cas system, "tCR...