A new support for the immobilization of β-d-galactosidase from Kluyveromyces lactis was developed, consisting mesoporous silica/titania with a chitosan coating. This presents high available surface area and adequate pore size optimizing efficiency enzyme and, furthermore, maintaining its activity. The obtained supported biocatalyst applied in hydrolytic activity tests o-NPG, showing 1223 Ug−1, ...

Although the lactose transport protein (M protein) is one of the most extensively studied membrane proteins, questions still remain with regard to the number of available transport sites in a fully induced cell. One approach to such a quantitative study has been the measurement of membrane binding of certain galactosides with high affinity for the carrier under conditions which prevent active t...

Abstract Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates not introduced feedstocks oleaginous yeasts. Results In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder c...

Milk is an important food substance and has a complex polydisperse system. The main components of milk consist lactose, protein, lipids. Mineral substances vitamins are also present in as minor nutrients. Lactose known sugar the only carbohydrate it consists glucose galactose. content lactose varied according to types. Moreover, most energy source during first years human life, providing almost...

Lactose and neuramin lactose were produced by incubating subcellular particles from rat mammary gland in a system containing glucose, uridine diphosphate galactose, and cytidine monophosphate N-acetylneuraminic acid. The K, values for glucose in the synthesis of neuramin lactose were fairly constant for three similarly prepared enzyme preparations (0.25, 0.17, and 0.22 m&r), but were widely dis...