Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total...

Abstract The aim of this review was to present various topics related lactose intolerance with special attention given the role fermented foods and probiotics in alleviating gastrointestinal symptoms. Lactose is a common digestive problem which human body unable digest lactose, known as milk sugar. can either be hereditary or consequence intestinal diseases. Recent work has demonstrated that da...

The ability of intravenously administered lactose in normal saline to prevent a decline in packed cell volume (PCV) during experimental trypanosomosis was studied in Zebu cattle. During the lactose infusion period, the PCV was stable up to Day 5 post-infection (p.i.) in a lactose-infused group, compared to that in an uninfused group in which the PCV dropped significantly (P < 0.05) as shown by ...

Milk is an important source of high quality protein, energy, calcium, potassium, phosphorus and riboflavin. It also has good functional properties and a highly acceptable taste, making milk a good alternative for the nutrition of children and for food programmes in developing countries. However, in some instances it has been advocated that milk or milk based products might not be appropriate fo...

A Lac+ mutant of Erwinia chrysanthemi was isolated from the Lac- wild type on lactose agar. beta-Galactosidase was expressed independently of lactose transport in both the mutant and the wild type, and neither strain expressed thiogalactoside transacetylase. Lactose transport and alpha-galactosidase, constitutive in the Lac+ strain, were coordinately induced in the Lac- strain by melibiose and ...

Aims: To evaluate whether orocoecal transit time (OCTT) might improve the correct allocation (lactose absorbers vs malabsorbers) of subjects with “borderline” H2 Breath test (H2BT) values ranging 10-20 ppm, and to determine among malabsorbers if OCTT can aid to discern lactose intolerant from tolerant individuals. Patients and Methods: OCTT and increment of H2 levels in breath following a dose ...

Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.

Background: The prevalence of genetically determined lactase non-persistence is based on the results of the lactose H 2 breath test. This test, however, is an indirect test, which might lead to misinterpretation. Design: We determined lactase activity in healthy Chinese and Dutch students using a novel C-lactose digestion test. The cut-off value of this test was established in a Chinese populat...

This application note reports on the development of a calibration model, its validation and applicability for the determination of residual lactose in lactose-reduced milk and milk products using a Lactoscope FTIR Advanced (FTA) Instrument. The calibration model developed for milk was based on a set of 76 samples, including process samples, endproducts of various brands of skim milk, semi skim ...