Dušková M., Karpíšková R. (2013): Antimicrobial resistance of lactobacilli isolated from food. Czech J. Food Sci., 31: 27–32. Bacteria of the genus Lactobacillus are of great benefit in many areas of life. They are widely used in food industry, in particular as part of starter cultures for fermented dairy and meat products, but also in human and veterinary medicine as probiotics. The increasing...

J. Villena, M.G. Vizoso-Pinto, V. Rodriguez, H. Kitazawa, S. Salva and S. Alvarez Immunobiotic Research Group Reference Centre for Lactobacilli (CERELA-CONICET). Tucuman, Argentina. INSIBIO-CONICET, Biomedical Department, Faculty of Medicine, National University of Tucumán, Argentina. Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University. Sendai, Japan. Inst...

The displacement of Enterococcus faecalis 1131 from hydrophobic and hydrophilic substrata by isolates of Lactobacillus casei 36 and Streptococcus hyointestinalis KM1 was studied in a parallel plate flow chamber. The experiments were conducted with either 10 mM potassium phosphate buffer or human urine as the suspending fluid, and adhesion and displacement were measured by real-time in situ imag...

Aminotransferases and glutamate dehydrogenase are two main types of enzymes involved in the initial steps of amino acid catabolism, which plays a key role in the cheese flavor development. In the present work, glutamate dehydrogenase and aminotransferase activities were screened in twenty one strains of lactic acid bacteria of dairy interest, either cheese-isolated or commercial starters, inclu...

We studied the vaginal microflora of 73 postmenopausal women who had never received estrogen replacement therapy. The median age of these women was 67 years, and none of them had bacterial vaginosis. Lactobacilli were detected in 36 (49%) of these women at a mean concentration of 10(5.7) cfu/g of vaginal fluid. H2O2-producing lactobacilli were recovered from 38% of the women. Some of the other ...

Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for 6 mucosal immunization against porcine parvovirus (PPV) infection. A 64kDa fragment of PPV 7 major protective antigen VP2 protein was used as the parvovirus antigen model. A 8 recombinant lactobacilli expressing VP2 protein was constructed with plasmid pPG611.1, 9 where expression and localization of the VP2 protein fr...

A previous communication (2) indicated that heme-linked enzymes were not involved in the very considerable capacities of various representative microaerophilic lactobacilli for electron transport from a variety of oxidizable substrates to oxygen, but that specific flavin components were present in high concentration in each of these organisms. To determine the possible central role of flavin en...

Forty-one strains of Bifidobacterium and Lactobacillus were tested for their ability to induce the proliferation of murine splenocytes using the MTT assay. The tested bifidobacteria induced the apparent proliferation of the murine splenocytes in a strain-dependent manner, while non of the tested lactobacilli showed any mitogenic activity. The bifidobacteria from allergic infants stimulated the ...

Succinate was the major organic acid detected in the hindgut content of pigs suffering from antibiotic-associated diarrhea. Antibiotic-associated diarrhea was induced by an oral dose of polymyxin B sulfate (3,000,000 units/day) or an intramuscular injection of enrofloxacin (0.6 g enrofloxacin/day). In the large intestine of enrofloxacin-treated pigs, Gram-negative facultative anaerobic rods phy...

In a study of minimal requirements for certain lactobacilli, Leuconostoc dextranicum 8086 was observed to require either aspartic acid or threonine for growth in a particular medium. Since such an observation does not allow specific conclusions regarding the relationship of these two amino acids, a study of this interrelationship in lactobacilli in which aspartic acid biosynthesis can be contro...