Alginate-gelatin mixed gel was applied to immobilized laccase for decolorization of some synthetic dyes including crystal violet. The immobilization procedure was accomplished by adding alginate to a gelatin solution containing the enzyme and the subsequent dropwise addition of the mixture into a stirred CaCl(2) solution. The obtained data showed that both immobilized and free enzymes acted opt...

Enzyme application in biotechnological and environmental processes has had increasing interest due to its efficiency, selectivity and mainly for being environmentally healthful, but these applications require a great volume of enzymes. In this work the effect of different concentrations of ethanol and 2,5-xylidine on growth and production of laccase by Pycnoporus sanguineus was investigated. In...

The oxidation of oleuropein and 3-hydroxytyrosol by oxidases laccase, tyrosinase, peroxidase has been studied. use a spectrophotometric method another chronometric made it possible to determine the kinetic parameters Vmax KM for each enzyme. highest binding affinity was shown laccase. antioxidant capacities these two molecules have characterized, finding very similar primary capacity between th...

In this study, Fe3O4/CMC magnetic nanoparticles were synthesized through co-precipitation method. Afterward, laccase from Trametes hirsuta was immobilized onto Carboxymethyl cellulose (CMC)-coated magnetic Fe3O4 nanoparticles by covalent bonding between carboxyl groups of carboxymethyl cellulose and amine group of laccases. Also, the...

The current study includes isolation of laccase producing fungus from compost soil and characterization of the laccase enzyme. In our study, 11 fungal isolates were isolated by serial dilution. They were cultivated on potato dextrose agar (PDA) plate with indicator compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) to screen for the laccase production ability. Out of 11 iso...

Laccase enzymes are now commercially available, and a laccase/mediator combination is currently marketed for indigo dye bleaching in textile manufacturing; replacing traditional chemical-based processes with enzymatic technology reduces the need for effluent treatment. However, an inexpensive source of these enzymes will be needed to enable wider application of this technology. In the present w...

The present study focused on laccase production, characterization, and its involvement in chlorophyll decolorization. Extracellular laccase, with the highest activity of 11 U/ml on day 8, was efficiently produced from Trametes hirsuta in 5 l bioreactor with optimized media comprising dual carbon sources, glucose and water hyacinth. A laccase was then purified from the supernatant to homogeneity...

Cryptococcosis is a life-threatening infection in humans and animals caused by encapsulated yeasts of the genus Cryptococcus. Cryptococcus neoformans and Cryptococcus gattii are the main agents of this mycosis. Until 2002 C. gattii was classified as a variety of C. neoformans but now is accepted as an independent species. The laccase (phenoloxydase) enzyme produced by these yeasts is considered...

Abstract Azo dyes released by the textile industries cause severe damage to environment and living organisms. The degradation of azo is widely studied using enzymatic methods. Laccase a copper-containing enzyme that degrades into less toxic compounds. In this work, crude laccase produced alkaliphile Pseudomonas mendocina in mixed dye showed 0.386 U/mL activity at pH 8.5. A combination green syn...