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1. Photoinduced generation of electric current by bacteriorhodopsin, incorporated into the planar phospholipid membrane, has been directly measured with conventional electrometer techniques. 2. Two methods for bacteriorhodopsin incorporation have been developed: (a) formation of planar membrane from a mixture of decane solution of phospholipids and of the fraction of violet fragments of the Hal...
Four ryanodine receptor type 1 and 2 chimeras (R4, R9, R10, and R16) and their respective wild-type ryanodine receptors (type 1 and 2; wtRyR1 and wtRyR2) were expressed in dyspedic 1B5 to identify possible negative regulatory modules of the Ca2+ release channel that are under the influence of the dihydropyridine receptor (DHPR). Responses of intact 1B5 myotubes expressing each construct to caff...
1. Intracellular recordings were made from the myoepithelial cells of the proventriculus of the marine polychaete worm Syllis spongiphila. Over-shooting responses were elicited either by carbamylcholine added to the bathing medium or by directly applied intracellular current pulses. 2. In control artificial sea water (ASW) directly applied current pulses elicited regenerative responses of 68-11...
Kajiya, Hiroshi, Fujio Okamoto, Hidefumi Fukushima, Keisuke Takada, and Koji Okabe. Mechanism and role of high-potassium-induced reduction of intracellular Ca2 concentration in rat osteoclasts. Am J Physiol Cell Physiol 285: C457–C466, 2003. First published April 9, 2003; 10.1152/ajpcell.00033.2003.—Osteoclasts are multinucleated, bone-resorbing cells that show structural and functional differe...
BACKGROUND An increase in the frequency of circulating endothelial colony forming cells (ECFCs), the only subset of endothelial progenitor cells (EPCs) truly belonging to the endothelial phenotype, occurs in patients affected by primary myelofibrosis (PMF). Herein, they might contribute to the enhanced neovascularisation of fibrotic bone marrow and spleen. Store-operated Ca2+ entry (SOCE) activ...
11. Outline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 12. Supplemental Appendix A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 12.1. Proof of Theorem 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 12.2. Proof of Theorem 2(a) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ...
We used fura-2 video imaging to characterize two Ca2+ influx pathways in mouse thymocytes. Most thymocytes (77%) superfused with hypoosmotic media (60% of isoosmotic) exhibited a sharp, transient rise in the concentration of intracellular free Ca2+ ([Ca2+]i). After a delay of approximately 70 s, these swelling-activated [Ca2+]i (SWAC) transients reached approximately 650 nM from resting levels ...
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