INTRODUCTION Along with introduction of dentin bonding agents (DBA), their clinical use as lining materials is increasing rapidly. Since remaining dentinal thickness (RDT) has always been a concern for cytopathic effect of restorative materials, its effect on reduction of cytotoxicity of these materials especially DBAs is critical. The purpose of this study was to evaluate and compare the cytot...

INTRODUCTION The purpose of the current study was to estimate the biocompatibility of endodontic sealers with different bases on L929 mouse fibroblasts permanent cell line using Multiscan EX Spectrophotometer. MATERIALS AND METHODS Endodontics sealers used in this study were GuttaFlow (Roeko) silicone based sealer, AH plus (De Tray-DENTSPLY) epoxy resin based, Apexit (Vivadent) calcium hydrox...

After attachment to receptors, reovirus virions are internalized by endocytosis and exposed to acid-dependent proteases that catalyze viral disassembly. Previous studies using the cysteine protease inhibitor E64 and a mutant cell line that does not support reovirus disassembly suggest a requirement for specific endocytic proteases in reovirus entry. This study identifies the endocytic proteases...

The mechanism of augmentation of tumor cell killing by immune effector cells and chemotherapeutic drugs was studied. The effect of treating tumor cells with various antineoplastic drugs on their sensitivity to murine natural cell-mediated cytotoxicity in vitro was investigated. Pretreatment with actinomycin D at nontoxic concentrations rendered L929 and WEHI-164 tumor cells more susceptible to ...

The tumour cell lines U937A and L929 form large, loosely packed colonies in vitro and can be killed by the cytokine tumour necrosis factor (TNF).

Both hyaluronidase and transforming growth factor (TGF)-β1 play a significant role in the development of prostate cancer. In this study, the regulation of tumor necrosis factor (TNF)-mediated cell death by hyaluronidase and TGF-β1 was investigated. Preexposure of L929 fibroblasts, prostate LNCaP cells, and epithelial Mv 1 Lu cells to hyaluronidase for a minimum of 12 h resulted in significant e...

Serine palmitoyltransferase (SPT) plays the key role on catalysing formation of 3-ketodihydrosphingosine, which is first step de novo biosynthesis sphingolipids. SPT linked to many diseases including fungal infection, making it a potential therapeutic target. Thus, logical docking-based virtual screening method was used screen selective inhibitor against fungi, not human. We myriocin-similarity...

Low energy plasma has been introduced to treat the surface of Thai silk fibroin which should be enhanced for cell adhesion due to its native hydrophobic surface. Plasma surface treatment could introduce desirable hydrophilic functionalities on the surface without using any chemicals. In this work, nitrogen glow discharge plasma was generated by a low energy AC50Hz power supply system. The plasm...

introduction : the aim of this study was to compare the cytotoxicity of mineral trioxide aggregate (mta) and a new endodontic cement (nec) on l929 mouse fibroblasts. materials and methods: different dilutions (neat, 1/2, 1/10, 1/100) of fresh and set materials placed adjacent flasks of l929 in dmem medium. cellular viability was assessed using mtt assay in three time intervals (24, 48, and 72 h...