BACKGROUND To study the molecular characteristics of a long-term, low frequency outbreak of bla KPC-2 in a low prevalence setting involving the hospital environment. METHODOLOGY/PRINCIPAL FINDINGS KPC-producing bacteria were screened by selective chromogenic agar and Real-Time PCR. The presence of antibiotic resistance genes was ascribed by PCRs and subsequent sequencing, and the KPC-producin...

We analyzed the whole genome sequence and resistome of the outbreak Klebsiella pneumoniae strain MP14 and compared it with those of K. pneumoniae carbapenemase- (KPC-) producing isolates that showed high similarity in the NCBI genome database. A KPC-2-producing multidrug-resistant (MDR) K. pneumoniae clinical isolate was obtained from a patient admitted to a Korean hospital in 2011. The strain ...

We present new VLA C-configuration H i imaging of the Lyα-emitting starburst galaxies Tol 1924−416 and IRAS08339+6517. The effective resolution probes neutral gas structures larger than 4.7 kpc in Tol 1924−416, and larger than 8.1 kpc in IRAS08339+6517. Both systems are revealed to be tidally interacting: Tol 1924−416 with ESO338−IG04B (6.6 = 72 kpc minimum separation), and IRAS08339+6517 with ...

We have used the Australia Telescope Compact Array (ATCA) and the Swedish-ESO Submillimetre Telescope (SEST) to map the large-scale atomic and molecular gas in the nearby (4 Mpc) Circinus galaxy. The ATCA H I mosaic of Circinus exhibits the warps in position angle and inclination revealed in the single-pointing image of Jones et al. (1999), both of which appear to settle beyond the inner 30 kpc...

OBJECTIVES An extremely drug-resistant (XDR) clone of KPC-3-producing Klebsiella pneumoniae emerged in Israel in 2006, causing a nationwide outbreak. We aimed to characterize the local KPC-3-encoding plasmid carried by these isolates and study its contribution to antibiotic resistance. METHODS Mechanisms of carbapenem resistance were investigated in seven selected isolates (isolated between 2...

BACKGROUND Bacterial resistance to antibiotics has become a major source of concern for public health. Pseudomonas aeruginosa strains are important opportunistic pathogens. These bacteria have a high resistance to a wide range of existing antimicrobials and antibiotics. OBJECTIVES The present study was performed to evaluate the frequency of KPC in P. aeruginosa isolated from clinical samples ...

The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in our hospital increased beginning in 2009. We aimed to study the clinical and molecular epidemiology of these emerging isolates. We performed a retrospective review of all adult patients with clinical cultures confirmed as CPE by positive modified Hodge test from 5/2009- 5/2010 at the University of Michigan Health System (UMHS...

From June to September 2011, a total of 305 ertapenem-nonsusceptible Enterobacteriaceae isolates (MICs of ertapenem ≥ 1 μg/ml) were collected from 11 hospitals in different parts of Taiwan. The MICs of 12 antimicrobial agents against these isolates were determined using the broth microdilution method, and genes for carbapenemases were detected using PCR. Genotypes of isolates possessing carbape...

Background. We aimed to study the molecular characteristics and clonal structure of carbapenemase-producing Enterobacteriaceae(CPE) in post-acute care facilities (PACF’s) in Israel and to analyze the temporal changes that have occurred between 2008 and 2013. Methods. The prevalence of CPE carriage in PACF’s in Israel was determined in two point prevalence national surveys done in 2008 and 2013....