We describe a radioimmunoassay for lysozyme (EC 3.2.1.17, mucopeptide N-acetylmuramohydrolase) in plasma and urine. It involves use of an antibody, raised in rabbits, to lysozyme from urine from a patient with monocytic leukemia. This antiserum, diluted 4000-fold, is incubated with radiolabeled lysozyme for 2 h and antibody-bound lysozyme is separated from free lysozyme with dextran-coated char...

Davie, Joseph M. (Indiana University, Bloomington), and Thomas D. Brock. Action of streptolysin S, the group D hemolysin, and phospholipase C on whole cells and spheroplasts. J. Bacteriol. 91:595-600. 1966.-The effect of streptolysin S, the group D hemolysin, and phospholipase C (the alpha toxin of Clostridium perfringens) on whole cells and spheroplasts or protoplasts of three strains of strep...

Marine sponges often harbor dense and diverse microbial communities including actinobacteria. To date no comprehensive investigation has been performed on the culturable diversity of the actinomycetes associated with South China Sea sponges. Structurally novel aromatic polyketides were recently discovered from marine sponge-derived Streptomyces and Saccharopolyspora strains, suggesting that spo...

Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%; lipids, 30%; and polyphosphate, 27%. This fraction also contained small amou...

Arithmetic linear growth has been defined by Fisher et al. (1951) as a prolonged growth phase in which bacterial density increases as a straight line function of time. Fisher and Kirchheimer (1952) found this type of growth in 15 different strains of mycobacteria and proposed that arithmetic linear growth is a characteristic feature of members of the genus Mycobacterium. They suggested this phe...

In previous studies it was found that the growth of 110 of 169 strains of bacteria tested was prevented by human saliva (Bibby, Clough and Hine, 1938). This effect was more marked against some types of organisms than against others, those most affected being bacilli and cocci isolated from air and water; those least affected, organisms isolated from the mouth. Differences occurred in the inhibi...

We describe three new species of springsnails (genus Pyrgulopsis) from the Amargosa River basin, California and Nevada (P. licina sp. n., P. perforata sp. n., P. sanchezi sp. n.), each of which was previously considered to be part of P. micrococcus. We also restrict P. micrococcus to its type locality area (Oasis Valley) and redefine a regional congener, P. turbatrix, to include populations fro...

Studies on the process of nitrite reduction by cell-free extracts of denitrifying bacteria could materially contribute to our knowledge of its mechanism. Such investigations have so far been limited to those of Yamagata (1) with Bacillus pyocyaneus (Pseudomonas aeruginosa) and of Robinson et al. (‘2) with Micrococcus haZodenitri$cans. Neither of these authors has examined the products of the re...