Human embryonic stem cells are pluripotent cells derived from the inner cell mass of the blastocyst. Once isolated in culture, they can produce stable cell lines with the capacity to provide differentiated cells from all three germ layers. This ability is the centre of an emerging field of research into applications ranging from tissue engineering and drug discovery to developmental biology and...

Cryopreservation of human umbilical vein endothelial cells (HUVECs) facilitated their commercial availability for use in vascular biology, tissue engineering and drug delivery research; however, the key variables in HUVEC cryopreservation have not been comprehensively studied. HUVECs are typically cryopreserved by cooling at 1 °C/min in the presence of 10% dimethyl sulfoxide (DMSO). We applied ...

The effects of three fungicide seed treatments (triazole plus imidazole (tri+im), fuberidazole (fub) and carboxin plus thiram (car+thi)) on establishment and yield of a number of paired seed lines of wheat cultivars with different levels of Fusarium infection were examined. Without seed treatment, lines of four cultivars with an average of 48% of seeds infected with Fusarium had between 9% and ...

For the purpose of opinion exploring in tweets, this article presents a sentiment classification of tweets content. First, we present a method to identify new sentiment similarity seed words. These seed words are used for predicting sentiment intensity of other words and short phrases in co-occurrence. Then, for testing sentiment similarity, we use: Similarity Measures methods between words and...

In this paper we solve some open problems related to (pseudo)palindrome closure operators and to the infinite words generated by their iteration, that is, standard episturmian and pseudostandard words. We show that if θ is an involutory antimorphism of A, then both θ-palindromic closures of any factor of a θ-standard word are also factors of some θ-standard word. We also introduce the class of ...

Abstract The objective of this study was to evaluate the efficiency cryoprotective solution (PVS2) combined with phloroglucinol for cryopreservation seeds two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds had 91.03% initial viability 91.99% germination. treatment PVS2 at 0 °C 1% 60 min returned 93.79% 91.01% germination after recovery from LN, consequently resulting in faster...

Approaches to determining the factuality of diagnoses and findings in clinical text tend to rely on dictionaries of marker words for uncertainty and negation. Here, a method for semi-automatically expanding a dictionary of marker words using distributional semantics is presented and evaluated. It is shown that ranking candidates for inclusion according to their proximity to cluster centroids of...

The field of cellular therapeutics has immense potential, affording an exciting array of applications in unmet medical needs. One of several key issues is an emphasis on getting these therapies from bench to bedside without compromising safety and efficacy. The successful commercialization of cellular therapeutics will require many to extend the shelf-life of these therapies beyond shipping "fr...

Ovarian tissue cryopreservation is the key step towards the establishment of an ovarian tissue bank or the preservation of ovarian tissue for patients scheduled for gonadotoxic cancer therapies, aiming for fertility restoration later on. Conventional cryopreservation, or slow freezing, has been the mainstay of ovarian tissue cryopreservation. Vitrification has recently emerged as a new trend fo...