Laponite is a nanoplatform that has been successfully used as new biomaterial for drug delivery, tissue engineering and bioimaging at the nanoscale. In general, deep knowledge of mechanism interaction nanomaterial with biological components in physiological environment highly desirable properly characterizing its therapeutic efficacy toxicology. Up to know, use fluorescent dyes labelling both, ...

In continuation our effort to research the chemical space of aryloxy-naphthoquinones as potential anti-Chagas agents, we synthesized nine derivatives and these compounds were evaluated in vitro against epimastigote trypomastigote forms Mexican strains Trypanosoma cruzi (T. cruzi). Most are highly active (IC50 < 1.0 µM) compared reference drug benznidazole (Bzn). Then on trypomastigotes, which i...

Listeriolysin O, the major virulent determinant of Listeria monocytogenes, is known for forming pores on cholesterol-rich membranes. In the present study, we reveal its other facet, rafts clustering. By immunofluorescence microscopy, we show that the glycosylphosphatidylinositol-anchored proteins CD14 and CD24, which normally exhibit uniform distribution on J774 cells, undergo clustering upon t...

This study reports the synthesis and antioxidant activity of some new acetamide derivatives. The compounds' structures were elucidated by NMR analysis and their melting points were measured. The in vitro antioxidant activity of these compounds was tested by evaluating the amount of scavenged ABTS radical and estimating ROS and NO production in tBOH- or LPS-stimulated J774.A1 macrophages. All co...

Experimental chronic (45-day-old) skin lesion in hamster hind foot induced by Leishmania (Viannia) lainsoni infection showed the presence of promastigote forms in the tissue, inside parasitophorous vacuoles, as assessed by transmission electron microscopy. Experimental in vitro interaction (24 and 48 h) between Leishmania (V.) lainsoni and J774-G8 macrophage cells also demonstrated the same pro...