Ribonucleotide reductase is an essential enzyme in mammalian DNA replication. In quiescent BHK-21/C13 cells exhibiting a low level of ribonucleotide reductase activity, infection with herpes simplex virus (HSV) resulted in the early induction of an altered ribonucleotide reductase. The extent of the induction was dependent upon the m.o.i. and could be diminished or prevented by u.v. treatment o...

BACKGROUND & OBJECTIVES Anopheles stephensi and A. culicifacies are the two major vectors of malaria in Karnataka. These mosquito populations are continuously being exposed directly or indirectly to different insecticides including the most effective pyrethroids. Therefore, there is a threat of insecticide resistance development. We subjected these vectors to larval bioassay using two popular p...

The principal cytosol thymidine kinase activity of African green monkey kidney cells increased approximately threefold at 2I to 29 h after infection with adenovirus type 5. Disc polyacrylamide gel electrophoresis (disc PAGE) analyses showed that the electrophoretic mobility relative to the tracking dye (Rm) of the cytosol thymidine kinase from both mock-infected and adenovirus type 5-infected c...

The M4 isozyme of lactate dehydrogenase was purified to homogeneity from normal rat liver and from two Morris hepatomas (7777 and 7793). Amino-terminal analyses with fluorodinitrobenzene failed to detect the presence of free amino-terminal residues in each enzyme studied. Each enzyme contained between 3.7 and 4.1 moles of protein-bound acetyl groups per mole of enzyme. The amino-terminal peptid...

Asian papaya fruit fly, Bactrocera papayae Drew & Hancock and carambola fruit fly, Bactrocera carambolae Drew & Hanckock (Diptera: Tephritidae) are the major agricultural pests, especially fruits and vegetables, in Malaysia. A vertical type polyacrylamide gel electrophoretic technique was used for the first time to compare the esterase isozyme banding patterns between the larvae of Bactrocera p...

Live or frozen insects are required for using isozyme and DNA RFLP methods in studies on population structure, systematics and incrimination of sibling species. Difficulty in keeping insects alive or unavailability of liquid nitrogen or dry ice at regular intervals during extended collection trips poses a serious problem. We describe a method for preserving insects in hexane, under field condit...

The particulate and soluble fractions of cell-free extracts from seeds, roots, and leaves of 10 different plants were examined electrophoretically for isozymes of malate dehydrogenase. Distinct isozyme patterns were observed for each plant and even for the individual tissues of each species. There were some isozymes in several different plant extracts with equal electrophoretic mobilities, but ...

To determine whether long-term reductions in neuromuscular activity result in alterations in metabolic capacity, the activities of oxidative, i.e., succinate dehydrogenase (SDH) and citrate synthase (CS), and glycolytic, i.e., alpha-glycerophosphate dehydrogenase (GPD), enzyme markers were quantified in rat soleus muscles 1, 3, and 6 mo after a complete spinal cord transection (ST). In addition...