Obvious lack of interfibrillar mitochondria (IFM) sub population in isolated mitochondria attributed to limitations in isolation procedure. In this manuscript, we compared the functional activities of mitochondrial sub-populations namely, IFM and sub-sarcolemmal (SSM) mitochondria obtained from isolated rat heart subjected to different perfusion time namely (minutes) 0.5,5,10,25,60 and 120. Pro...

1. We have investigated the effects of ethanethiol, methanethiol and dimethyl sulphide on some metabolic processes of isolated rat hepatocytes, isolated mitochondria from liver and brain and ox-heart submitochondrial particles. 2. Ethanethiol, but not dimethyl sulphide, inhibited both gluconeogenesis and ureogenesis from various substrates in rat hepatocytes, depressed cellular ATP content and ...

Myocardial infarction is a common presentation of coronary artery disease and the leading cause of death worldwide. The present study was investigated potential resistance to ischemia-reperfusion (I/R) injuries following administration of methanolic (MeOH) extract of Scrophularia frigida (S. frigida) in isolated rat heart. Male Wistar rat hearts (n= 8-10) were isolated and allowed to equilibrat...

objective: rosa damascena mill l. (r. damascena) is a well-known plant with fragrant effects. several therapeutic effects of this plant on respiratory, gastrointestinal and nervous systems have been reported. it is also suggested to have beneficial effect on cardiovascular system especially blood pressure regulation. the present study was carried out to evaluate acute cardiovascular effect of h...

An antiserum with specific reactivity against the contractile proteins of the rat heart has been raised in the rabbit. Fractions of the serum have been shown to enter the isolated rat embryo by electron-microscopic studies with ferritin, where they bind to the myocardium, producing degenerative changes. Both IgG and IgM fractions are toxic, producing foetal death in a large proportion of explan...

2. Scheuer J, Stezoski SW: Effect of high energy phosphate depletion and repletion on the dynamics and electrocardiogram of isolated rat hearts. Circ Res 23: 519 530, 1968 3. Rovelto M J, Whitmer JT, Neely J R: Comparison of the effects of anoxia and whole heart ischemia on carbohydrate utilization in isolated working rat hearts. Circ Res 32: 699-711, 1973 4. Williamson JR: Glycolytic control m...

this study intended to assess the efficacy of acute administration of natural honey on cardiac arrhythmias and infarct size when it is used during the normothermic ischemia in isolated rat heart. during 30 min of regional normothermic ischemia followed by 120 min of reperfusion, the isolated hearts were perfused by a modified drug free krebs-henseleit solution (control) or the solution containi...

Phenformin causes lactic acidosis in clinical situations due to inhibition of mitochondrial respiratory chain complex I. It is reportedly taken up by hepatocytes and exhibits mitochondrial toxicity in the liver. In this study, uptake of phenformin and [(14)C]tetraethylammonium (TEA) and complex I inhibition by phenformin were examined in isolated liver and heart mitochondria. Uptake of phenform...

Knowledge of the rate of development of immature cardiomyocytes after implantation into a host heart is important for studies using cell therapy. To assess this functionally, we have implanted rat neonatal cardiomyocytes (NCMs) in normal and infarcted rat heart and re-isolated them for functional assessment. Maturation of implanted bone marrow stromal cells (BMSCs) was compared under similar co...

The relation between myocardial tissue cyclic AMP (cAMP) and the vulnerability to ventricular fibrillation was assessed in the isolated perfused rat heart by measurement of ventricular fibrillation threshold (VFT) and vulnerable period duration (VP). Exogenous dibutyryl cyclic AMP (DBcAMP) reduced VFT and increased VP by a concentration-related action whereas exogenous cAMP did not. Theophyllin...