Transgenesis in numerous eukaryotes has been facilitated by the use of site-specific integrases to stably insert transgenes at predefined genomic positions (landing sites). However, the utility of integrase-mediated transgenesis in any system is constrained by the limited number and variable expression properties of available landing sites. By exploiting the nonstandard recombination activity e...

introduction: ankle fracture is one of the most common joint fractures. x-ray and physical examination are its main methods of diagnosis. recently, ultrasonography (us) is considered as a simple and non-invasive method of fracture diagnosis. this study evaluated the diagnostic accuracy of us in detection of ankle fracture in comparison to plain radiography. methods: in this diagnostic accuracy ...

background: we aimed to extract the histogram features for text analysis and, to classify the types of bio medical waste (bmw) for garbage disposal and management. methods: the given bmw was preprocessed by using the median filtering technique that efficiently reduced the noise in the image. after that, the histogram features of the filtered image were extracted with the help of proposed modifi...

background: diagnosis of food allergy is difficult in children. food allergies are diagnosed using several methods that include medical histories, clinical examinations, skin prick and serum-specific immunoglobulin e (ige) tests, radio-allergosorbent test (rast), food challenge, and supervised elimination diets. in this study we evaluated allergies to cow's milk, egg, peanut, and fish in c...

The broaden application of adoptive T-cell transfer has been constrained by the technical abilities to isolate and expand antigen-specific T cells potent to selectively kill tumor cells. With the recent progress in the design and manufacturing of cellular products, T cells used in the treatment of malignant diseases may be regarded as anticancer biopharmaceuticals. Genetical manipulation of T c...

To demonstrate the utility of phage display in generating highly specific antibodies, affinity selections were conducted on 20 related Src Homology 2 (SH2) domains (ABL1, ABL2, BTK, BCAR3, CRK, FYN, GRB2, GRAP2, LYN, LCK, NCK1, PTPN11 C, PIK3R1 C, PLCgamma1 C, RASA1 C, SHC1, SH2D1A, SYK N, VAV1 and the tandem domains of ZAP70). The domains were expressed in Escherichia coli, purified and used i...

Structural characterization of oligosaccharide products generated by enzymatic hydrolysis of plant cell wall polysaccharides provides valuable information about the enzyme's activity and substrate specificity. In this chapter, we describe some of the chemical, chromatographic, and spectroscopic methods that we routinely use to isolate and characterize oligosaccharides formed by enzymatic fragme...

Neutralization of Chlamydia trachomatis was assayed by the decrease in inclusion-forming units in baby hamster kidney cells grown in culture. Five percent fresh guinea pig sera increased neutralization titers of rabbit antisera 100- to 1,000-fold but had no effect when normal rabbit sera were tested. Neutralization of a type A or B trachoma isolate was strain specific. Neutralization by human e...

Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with the co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, high specificity reagents to target and capture proteins of interest along with specifically associated proteins from cell extracts. Affinity capture coupled to mass spe...