The objective of the present investigation was to develop a novel, accurate, precise, and linear High-Performance Liquid Chromatographic (HPLC) method for simultaneous estimation p-Cymene aloe-emodin in novel topical herbal formulation validated as per ICH guidelines. In current study, good chromatographic separation achieved isocratic mode using an HPLC C18 column (250mm × 4.6), 5 μm, mobile p...

The aim of this work was to perform an extended stability study for the analgesic containing fentanyl, clonidine and ropivacaine in physiological saline solution 0.9% at different infusion sites, such as bags, epidural sets syringes. assessed by HPLC system equipped with a photodiode array detector set 210 nm. separation conducted on C18 column maintained 40˚C using isocratic mobile phase consi...

Objective: An assay method was developed and validated for the simultaneous estimation of Lercanipine HCl atenolol using RP-HPLC. Methods: effective chromatographic separation achieved waters symmetry C18 column dimensions 150x4.6 mm, 3.5 μm, as a stationary phase. 0.1 percent ortho phosphoric acid acetonitrile in 50:50 v/v used mobile process with rate flow 1 ml/min UV detection carried out at...

A simple, fast, and sensitive method for the extraction through direct saponification, separation, quantification, and identification of 12 cholesterol oxidation products (COPs) and cholesterol in a single isocratic, normal-phase, high-performance liquid chromatography (HPLC) was developed. Three detectors were compared for determination of COPs and cholesterol in fish samples: refractive index...

A reverse phase high performance liquid chromatography method was developed for simultaneous estimation of nitazoxanide and ofloxacin in tablet formulation. The separation and quantification was achieved by Hiq Sil C(18)V Size 4.6 mm Ø (*)250 mm column in isocratic mode, with mobile phase consisting of acetonitrile-methanol-0.4 M citric acid, (60:30:10, v/v/v). Citric acid used to stabilize nit...

An analytical method based on solid phase extraction has been developed and validated for analysis of amoxicillin in human plasma using gemifloxacin as an internal standard. A COSMOSIL 5C18-PAQ column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatographic conditions and mass spectrometric detection in th...

PURPOSE A simple stability-indicating RP-HPLC assay method was developed and validated for quantitative determination of Chloramphenicol, Dexamethasone Sodium Phosphate and Tetrahydrozoline Hydrochloride in ophthalmic solution in the presence of 2-amino-1-(4-nitrophenyl)propane-1,3-diol, a degradation product of Chloramphenicol, and Dexamethasone, a degradation product of Dexamethasone Sodium P...

Overview Purpose: Methods that offer both rapid and sensitive measurement of amino acids are essential to neurochemical research. The ability to decrease analysis time enables an improvement in temporal resolution when studying amino acid neurotransmission. Methods: Two methods using pre-column derivatization were developed: first, a fast isocratic method with electrochemical detection for the ...

This study describes a simple, easy and inexpensive method for extracting tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza by sonication or decoction. A comparison of sonication and decoction showed a similar yield for cryptotanshinone, while the tanshinone IIA yield was slightly higher using sonication. An analytical method for detecting tanshinone IIA and cryptotanshinone in extra...

A normal-phase high-performance liquid chromatography (NP-HPLC) method for the determination of tocopherols and tocotrienols in hazelnuts is reported. Three extraction procedures (with and without saponification) were assayed; the best results were obtained with a simple solid-liquid extraction procedure. Chromatographic separation was achieved using an Inertsil 5 SI column using isocratic elut...