We present and validate an equilibrium model based on the Poisson-Boltzmann equations that includes main ingredients to simulate ion-sensitive membranes in context of electrochemical potentiometric sensors with without ionophores. With respect phase boundary models, our spatial resolution electrostatic potential concentrations. The enables study Nernstian non-Nernstian responses, helps improvin...

We conducted two experiments to evaluate the effect of the ionophore laidlomycin propionate (LP) on steer performance and ruminal N metabolism. Experiment 1 was a 91-d growth study evaluating the growth and ruminal characteristics of steer calves consuming supplemental LP. Steers (n = 96; 255 +/- 3 kg; four steers/pen; six pens/treatment) were used in a randomized complete block design with a 2...

Bovine epididymal spermatozoa incubated aerobically in vitro in the presence of 0.1 to 0.2 mM CaCl2 accumulate 25 to 50 nmol of calcium/10(8) cells. The addition of low concentrations of the ionophore A23187 (0.01 to 0.5 nmol/mg of sperm protein) induces efflux of this accumulated calcium. At high ionophore concentrations (0.5 to 5.0 nmol/mg of sperm protein), calcium release is followed by an ...

Cultured skin fibroblasts from patients with Duchenne muscular dystrophy (DMD) are more sensitive than normal cells to prolonged exposure to the ionophore monensin. In a cell spreading assay in which cells were preincubated with monensin and subsequently allowed to adhere to and spread on a glass substratum in serum-free medium for 100 min, the mean transformed cell area of normal and DMD cells...

Interest in polyether antibiotics has remained at a high level for over 20 years, owing largely to the commercial importance of this class of drugs in veterinary medicine. For example, monensin1*, lasalocid1} and salmomycin2) are marketed as anticoccidial agents for poultry, and are used as growth permittants in cattle or swine. Narasin1} and maduramicin3) are also used as anticoccidial agents....

Coccidiosis is likely the most costly disease threat to global poultry production and prevention of coccidiosis is essential in order to maintain intestinal integrity and the efficient absorption of nutrients in the chicken. Narasin is an ionophore anticoccidial. Ionophores transport cations such as sodium across the pellicle membrane of sporozoites of coccidia, resulting in a lethal osmotic im...

The binding affinity and selectivity of a new ionophore, [1(8)]starand (1), toward alkali metal cations in methanol were examined through NMR titration experiments and free energy perturbation (FEP) and molecular dynamics simulations. The preference was determined to be K(+) > Rb(+) > Cs(+) > Na(+) >> Li(+) in both FEP simulations and NMR experiments. The FEP simulation results were able to pre...

Background: Chemical activation is the most frequently method for artificial oocyte activation, resultant in high fertilization rates. The aim of this prospective randomized controlled study is evaluating the effect of calcium ionophore on fertilization and pregnancy rate in infertile men suffering from teratoospermia. Materials and Methods: 38 women with teratoospermic partner were divided int...