Hemoglobinopathies are the archetypal molecular disease, and from 1949, when Linus Pauling et al. first demonstrated that hemoglobin (Hb) S could be separated from Hb A by paper electrophoresis (1 ), the detection and identification of Hb variants has provided a testing ground for new methods of protein analysis. The actual molecular basis of sickle cell anemia was not finally elucidated until ...

Numerous experimental techniques for the separation of transfer ribonucleic acids have been used successfully in preparing partially purified fractions of several specific tRNAs.1 Many of the existing methods have depended upon the differential solubility of specific tRNAs in complex two-phase systems (I-B), and the separations were achieved by means of countercurrent extraction techniques. Col...

Numerous experimental techniques for the separation of transfer ribonucleic acids have been used successfully in preparing partially purified fractions of several specific tRNAs.1 Many of the existing methods have depended upon the differential solubility of specific tRNAs in complex two-phase systems (I-B), and the separations were achieved by means of countercurrent extraction techniques. Col...

Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we ...

The energetic ion distributions and confinements are studied in the LHD plasma applying a newly developed radial profile measurement system FICXS (Fast Ion Charge Exchange Spectroscopy) based on the Doppler shifted Balmer-alpha (Hα) emission from the energetic ions charge-exchanged with injected neutral beams. A clear difference is found in the Hα emission spectrum changing the magnetic configu...

Ion chromatography is a new analytical technique in which the ions to be analyzed are separated on a special pellicular ion-exchange column. Once separated, the effluent from the separating column passes into a second ion-exchange column, which removes the eluent or buffer ions from the moving phase without removing the sample ions, which are detected and measured by electrical conductivity on ...

Recent research trends in the development of ion exchange membranes and their use in separation processes with chemical reactions are reviewed. Emphasis in research on the ion exchange membrane is trending toward analysis of micro-structure of the membranes and to development of new functionalized ion exchange membranes in response to industrial requirements. Separation processes with chemical ...

An economic comparison of reverse osmosis and ion exchange in Europe. In: Greig JA (ed.) Ion Exchange Development and Applications, pp. 58}66. Cambridge: Royal Society of Chemistry. Nolan J and Irving J (1984) The effect on the capacity of strong base anion exchange resins of the ratio of chloride to sulphate in the feed water. In: Naden D and Streat M (eds) Ion Exchange Technology, pp. 160}168...

The separation and purification of various elements by ion exchange chromatography takes advantage of the variation of the electrostatic bond energies of ions in solution. Thus, the two main controlling factors in ion exchange chromatography are the ionic charge (z) and the ionic radius (r). These two are combined in the ionic potential – z/r – that can be used to compare relative electrostatic...