In recent years, the study of extracellular vesicles (EVs) in context various diseases has dramatically increased due to their diagnostic and therapeutic potential. Typically, EVs are isolated vitro from cell culture primary cells or lines bodily fluids. However, these methods do not represent whole complexity an vivo microenvironment, fluids contain a high heterogeneous population since they o...

To clarify the effect of renal dysfunction on pharmacokinetics of the prokinetic agent metoclopramide (MCP), we administered intravenously 0.4 mg/kg MCP to healthy calves and calves subjected to right kidney vessel ligation (ligation) without or with a subsequent left nephrectomy (ligation plus removal). Plasma MCP concentration, glomerular filtration rate (GFR) and plasma prolactin level were ...

Most protocols for yeast subcellular fractionation involve the use of mechanical shear forces to lyse the spheroplasts produced by the enzymatic digestion of the Saccharomyces cerevisiae cell wall. These mechanical homogenization procedures often involve the manual use of devices such as the Dounce homogenizer, and so are very operator-dependent and, in consequence, lack reproducibility. Here, ...

Subcellular fractionation has been an important tool in investigating human eosinophil structure and function, including localizing of cytokine/chemokines within granules, investigating granule protein translocation and intracellular transport during eosinophil secretion, and studying secretory mechanisms of granules. The resolution of organelles obtained by subcellular fractionation was improv...

Recently, we performed a contrast-enhanced thoracic 16-channel multi-slice computed tomography (CT) with intravenous (i.v.) administration of iodixanol [Visipaque, dose 0.5 g I/kg body weight (bw)] in a 73-year old patient, suffering from multiple myeloma and chronic renal failure (calculated creatinine clearance of 23ml/min). Three days later, an unenhanced CT of the upper abdomen was performe...

We report a patient who developed subarachnoid hemorrhage (SAH) just after coronary angiography (CAG) with non-ionic contrast media (CM) and minimal dose of heparin. The 55-year-old man had a history of acute ST elevation myocardial infarction that had been treated with primary percutaneous coronary intervention and was admitted for a follow-up CAG. The CAG was performed by the transradial appr...