A sensitive assay is developed to assess the existence of an iodide channel in a fraction of solubilized membrane proteins. This step is critical when considering various procedures for purification of this channel. Sodium cholate is used as a detergent as it does not denature the iodide channel. A simple and rapid method involving gel-filtration chromatography is used simultaneously to remove ...

Binding of an iodide ion to horseradish peroxidase was studied by following the hyperfine-shifted proton nuclear magnetic resonance signals of the enzyme. For the enzyme in an iodide-free solution, the spectra of hyperfine-shifted methyl region were only slightly affected by varying pH. In the presence of iodide (200 mM), however, both chemical shifts and line widths of the heme peripheral 1- a...

BACKGROUND Perchlorate and thiocyanate interfere with iodide uptake at the sodium-iodide symporter and are potential disruptors of thyroid hormone synthesis. Perchlorate is a common contaminant of water, food, and human milk. Although it is known that iodide undergoes significant diurnal variations in serum and urinary excretion, less is known about diurnal variations of milk iodide levels. O...

The sorption affinity of iodide on organo-bentonite, modified with hexadecyl pyridinium (HDPy), was investigated the iodine–starch method coupled UV/Vis absorption spectroscopy. complex optimized in terms reaction time and sample compositions, based absorbance. batch experiment for organo-bentonites, using two different loading amounts HDPy, conducted to analyze influence equilibrium time, liqu...

UNLABELLED The thyroid transcription factors TTF-1 and Pax8 cooperate in the transcriptional activation of thyroid-specific genes such as thyroglobulin (Tg), thyroperoxidase (TPO), and sodium/iodide symporter (NIS). METHODS Dog TTF-1 (dTTF-1) and the human Pax8 (hPax8) gene were transfected in Morris hepatoma (MH3924A) cells to investigate (a) the possible visualization of functional protein-...

(CH3)3 . N. CH2. CH2-S-P(z=0) (OC2H5)2.I The first report of its clinical use as a miotic in the treatment of glaucoma came from Leopold, Gold, and Gold (1957). Krishna and Leopold (1959) reported their results in 110 eyes of 62 patients with glaucoma treated with it for continuous periods extending, in many cases, up to 2 years without surgical intervention. Becker, Pyle, and Drews (1959) repo...

Crystals of the one-dimensional organic-inorganic lead iodide-based compound catena-poly[bis-(piperazine-1,4-diium) [[tetra-iodido-plumbate(II)]-μ-iodido] iodide monohydrate], (C4N2H12)2[PbI5]I·H2O, were obtained by slow evaporation at room temperature of a solution containing lead iodide and piperazine in a 1:2 molar ratio. Inorganic lead iodide chains, organic (C4N2H12)(2+) cations, water mol...