We report the preparation and characterization of poly(N-isopropylacrylamide) (PNIPAAm) polymer brushes exhibiting controlled lateral variations in the patchiness of polymer chains. These gradients were achieved through an atom transfer radical polymerization (ATRP) grafting-from approach utilizing surfaces on which the spatial profile of the initiator density was carefully controlled. Initiato...

Polystyrene-silica core-shell composite particles are prepared by one-step emulsion polymerization with a nonionic initiator VA-086, solely stabilized by silica nanoparticles. The silica nanoparticles are successfully incorporated as the shell, likely due to the fact that the nanoparticles are thermodynamically favorable to adsorb and remain at the liquid-liquid interfaces during the emulsion p...

DNA replication initiation, which starts at specific chromosomal site (known as replication origins), is the key regulatory stage of chromosome replication. Archaea, the third domain of life, use a single or multiple origin(s) to initiate replication of their circular chromosomes. The basic structure of replication origins is conserved among archaea, typically including an AT-rich unwinding reg...

The synthesized of emeraldine-salt-polyaniline composites were using in situ chemical polymerization to form the ES-PANI-glucomannan and ES-PANI-cellulose batch, spray method. Polyaniline can be composed glucomannan cellulose matrics because they are abundant, cheap, environmentally friendly. Ammonium peroxodisulfate (APS) is used as an initiator for process oxidative aniline HCl HCOOH doping. ...

Inspired by the chemical synthesis of molecularly imprinted polymers, we demonstrated for first time, protein-target mediated enzyme-generated aptamers (EGAs). We prepared pre-polymerisation mixtures containing different ratios nucleotides, an initiator sequence and protein template incubated each mixture with terminal deoxynucleotidyl transferase (TdT). Upon purification rebinding EGAs against...

The migration and transport of polymerization initiators are problematic for commercially used polymerization procedures. For example, UV printing of packaging generates products with potentially harmful components that come in contact with food. Enlarging the size of the initiator is the only way to prevent contamination, e.g., by gas phase transport. In this manuscript, the synthesis and adva...

NADH oxidases (NOXs) play an important role in maintaining balance of NAD+/NADH by catalyzing cofactors regeneration. The expression of nox gene from Lactobacillus brevis in Escherichia coli BL21 (BL21 (DE3)) was studied. Two strategies, the high AT-content in the region adjacent to the initiation codon and codon usage of the whole gene sequence consistent with the host, obtained the NOX activi...

Our laboratory has cloned the cDNA (Sutter, T. R., Tang, Y. M., Hayes, C. L., Wo, Y.-Y. P., Jabs, E. W., Li, X., Yin, H., Cody, C. W. , and Greenlee, W. F. (1994) J. Biol. Chem. 269, 13092-13099) and gene (Tang, Y. M., Wo, Y.-Y. P., Jabs, E. W., Stewart, J. C., Sutter, T. R., and Greenlee, W. F. (1996) J. Biol. Chem. 271, 28324-28330) for human CYP1B1, a new member of the cytochrome P450 superf...

Among the various online shopping business models, one that specifically attracts the attention of Internet users is “online group buying. which is characterized by group negotiation benefits and online word-of-mouth effects. Extant online group buying research has examined topics covering pricing mechanisms, pricing models, sales performance, and online users’ motivation to participation. Wher...

It has been shown that two of the three adeno-associated virus type 2 capsid proteins, B and C, are synthesized from a single spliced transcript. Protein C arises from an AUG codon at nucleotide 2810, whereas protein B is initiated by a unique eucaryotic initiation codon (ACG) that lies 65 triplets upstream from the C origin. The third capsid component, protein A, is synthesized from a second s...