A new metabolic pathway of L-tryptophan, tentatively referred to as the side chain pathway, was demonstrated in Pseudomonas fluorescens (ATCC 29574), in which the initial reaction was catalyzed by tryptophan side chain oxidase (Takai, K., Ushiro, H., Noda, Y., Narumiya, S., Tokuyama, T., and Hayaishi, 0. (1977) J. Biol. Chem. 252,2648-2656). When the bacteria harvested in the late stationary ph...

Tang and Bonner (16) have described an indoleacetic acid (IAA) oxidase system in pea epicotyls, and also an IAA oxidase inhibitor (17). These findings have been confirmed and extended by others (5-8, 18) and an IAA oxidase has been found in several plants, including beans (18) and pineapple leaf tissue (9). The presence of an inhibitor of IAA oxidation has not previously been shown in the pinea...

A large portion of indoleacetic acid (IAA)-induced inhibition of excised root tips and virtually all such inhibition of intact roots are the result of IAA-dependent ethylene production. Under certain conditions an additional effect of IAA accounts for a small portion of the inhibition of excised root tips. Ethylene production in response to applied IAA is governed by the level of applied auxin ...

An efficient protocol was developed for high frequency plant regeneration from leaf explants of Ammi majus. Ammi majus L. (Apiaceae) is a herb of pharmaceutical as well as ornamental interest and used in the treatment of leucoderma. Since in Ammi majus the seed set and germination is poor attempts were made to propagate the plants through tissue culture. The callus from cotyledonary leaves obta...

Growth of an auxin-requiring soybean cotyledon callus tissue (Glycine max L., Merr. var. Acme) was promoted by tryptophan, tryptamine, indole, indoleacetamide and, to a very slight degree, anthranilic acid. When tryptophan-3-(14)C was supplied in the growth medium, labeled indoleacetic acid (IAA) was found in both the tissue and the medium. Medium, from which the cells had been removed, was als...

Partial hydrolysis of polygalacturonic acid with a purified alpha-1,4-endopolygalacturonase yielded oligogalacturonides and trace amounts of a series of modified oligogalacturonides. Three of the minor products were isolated and identified as oxidized oligogalacturonides possessing termini of galactaric acid. Oxidation of indole-3-acetic acid by peroxidases was activated by oxidized oligogalact...

Lettuce, which is being grown in ever increasing quantities, suffers from a number of nutritional disorders which have been the subject of considerable speculation and study. Much of the difficulty with lettuce is evidently the result of the type of forcinig culture which is used to obtain the most firm and succulent heads. Among the micro-nutrient elements, boron has been given considerable at...