Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bond. Conversion of Trp to IAA is not competed by indole. Labeli...

The functional processes and mutual benefits of the wild plant Moringa oleifera its rhizosphere microbiome were studied via metagenomic whole-genome shotgun sequencing (mWGS) in comparison with a bulk soil microbiome. results indicated high gene abundance four KEGG categories, “Cellular Processes”, “Environmental Information Processing”, “Genetic “Metabolism”, Most enriched enzymes rhizobacteri...

Indole-3-acetic acid (IAA), a phytohormone auxin, may be involved in insect gall induction. We previously proposed that the IAA biosynthetic pathway is Trp → indole-3-acetaldoxime indole-3-acetaldehyde (IAAld) or IAAld IAA. In this study, we surveyed galling sawfly enzymes responsible for rate-limiting steps using heterologous protein expression system and identified PonAAS2, an aromatic aldehy...

  Objective: This study aimed to assess the destructive effects of citric acid, lactic acid and acetic acid produced from the fermentation of foods on primary teeth enamel.   Methods: This in vitro, experimental study was conducted on 24 sound primary teeth. The teeth were polished with a fine abrasive paper under running water. Tooth pieces measuring 3 × 4 × 3mm were cut out of the teeth and s...

A method for quantifying indole-3-acetic acid (IAA) and its conjugates with the six amino acids, Ala, -Asp, -Ile, -Glu, -Phe and -Val, in rice (Oryza sativa) by using high-performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (HPLC-ESI-MS/MS) is described. Samples from the rice plant or callus were treated with 80% acetone in water containing 2.5 mM...

The amounts of volatile substances responsible for the malodor of human waste (feces and urine) obtained from the storage tank of a community waste-water treatment plant were determined. Thus far, there has been little systematic research on malodor-causing substances of human waste. These substances were collected using Tenax-TA, and their concentrations were determined by the usual thermal-de...

The effects of feeding of 6-propylthiouracil (6-PTU) and polyunsaturated fatty acids (PUFA) independently and in combination and administration (ip) of a single dose of triiodothyronine (T3) (2.5 microg/100 g body wt) along with feeding of 6-PTU and PUFA were studied in rat brain. Dopamine (DA), 5-hydroxytryptophan (5-HTP), serotonin (5-HT), 5-hydroxy indole acetic acid (5-HIAA), norepinephrine...