The immunofluorescence of immunoglobulins and complement components in kidney specimens taken at necropsy was investigated to determine the persistence of antigenicity of immune reactants. Of 74 consecutive necropsies, 12 cases had positive glomerular fluorescence. The pattern and intensity were followed up for up to 15 days. Along with necropsy specimens, tissue samples of normal looking kidne...

A new direct immunofluorescence reagent (Syva and Genetic Systems Inc) was evaluated for its ability to detect Neisseria gonorrhoeae in specimens from populations with a high prevalence of the infection. Gonorrhoea was diagnosed by culture in 45 of 105 (43%) urethral specimens from men and 17 of 90 (28%) urethral and 25 of 60 (42%) cervical specimens from women. In men the immunofluorescence te...

BACKGROUND The aims of this study were to compare the morphological, biochemical, and functional properties of reprogrammed bone marrow stem cell (BMSC)-derived arterial endothelial cells (AECs) and venous endothelial cells (VECs), following adenosine triphosphate (ATP)-stimulation in a mini pig animal model. MATERIAL AND METHODS Bone marrow aspiration was performed in six adult mini pigs. Harv...

Murine double minute clone 2 (MDM2), p14 alternate reading frame (p14arf), and nucleophosmin (NPM) regulate p53 activity. A total of 200 biopsies, including normal bronchial, pre-invasive and invasive tissues, were examined for changes in NPM, p14arf, MDM2 and p53 expression patterns by immunohistochemistry and immunofluorescence with confocal microscopy. NPM and p14arf displayed a diffuse nucl...

Simian virus 40 (SV40)-transformed monolayer cells were analyzed in situ by indirect immunofluorescence microscopy for the postulated cell surface location of SV40 T-antigen-related molecules. With antisera prepared against purified, sodium dodecyl sulfate-denatured SV40 T-antigen, positive surface staining was obtained when the cells had been treated with formaldehyde before immunofluorescence...

Direct immunofluorescence and direct immunoperoxidase staining were equally sensitive and specific for detection of herpes simplex virus antigen in lesion specimens, and each method showed 82% agreement with virus isolation results.

A template for securing blocks of agar onto glass slides is described. Use of the template allows efficient handling as well as thorough washing of agar blocks during the immunofluorescence staining process.

Introduction Overcoming the immunosuppressive microenvironment in HCC is a major challenge. Better understanding of cell specific contribution required to help boost current immunotherapy. Endothelial cells are gatekeeper for immune recruitment and we undertook RNA-sequencing (RNA-seq) purified human endothelium define its tumour HCC. Methods were isolated from liver tissue us...

By immunofluorescence staining, a specific surface antigen induced by Venezuelan equine encephalomyelitis virus was detected on L-929 cells. Formation of the antigen was independent of viral ribonucleic acid synthesis.

A novel method for sequential immunofluorescence staining, which, in combination with 3D image registration and segmentation, can be used to increase the number of antigens that can be observed simultaneously in single cells in tissue sections is presented. Visualization of more than one antigen by multicolor immunostaining is often desirable or even necessary, both for quantitative studies and...