Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as complement to the single skin test. We developed lateral flow immunochromatography (LFIC) rapid bTB detection based on use conjugate gold nanoparticles with recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 control line, we selected given it was m...

Serology is based on the use of antibodies to detect circulating antibodies (immunoglobulin) or antigens. Largely used in the diagnosis of infectious diseases, it is now often replaced by DNA or RNA detection of infectious agents by molecular biology techniques such as polymerase chain reaction (PCR, rt-PCR). However, antibody detection can have some advantages on PCR techniques and numerous di...

The objective of this review is to describe the clinical and epidemiological characteristics of adenoviral conjunctivitis, as well as to present a practical update on its diagnosis, treatment and prophylaxis. There are two well-defined adenoviral keratoconjunctivitis clinical syndromes: epidemic keratoconjunctivitis and pharyngoconjunctival fever, which are caused by different adenovirus seroty...

Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are fit for diagnosing EI although their sensitivity is not optimal. Here, we evaluated the performance of the newly developed RAD test using silver amplification immu...

Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatogra...