Enzyme-linked immunosorbent assay (ELISA) has been served as both the workhorse and gold standard in immunoassay for its high specificity, sensitivity, accuracy, despite known shortcomings limitations....

The Serodia-HCV Particle Agglutination (HCV-PA) for the detection of HCV antibodies was compared with the Enzyme Immunoassay Test (UBI HCV EIA) for possible in-house use. A total of 150 specimens were analysed using UBI HCV EIA and Serodia-HCV PA. Of these, 80 (53.3%) were both PA and EIA positive and 59 (39.3%) were negative by both techniques. Eleven sera (7.4%) were found to be EIA-positive ...

Parvovirus B19 infection can cause severe effects in high-risk groups including pregnant women and immunocompromised individuals. Although serological detection of B19 infection is commonplace, minimal information is available on the absolute performance characteristics of various tests for the detection of B19 IgM. The performance of the first parvovirus B19 IgM enzyme immunoassay to be cleare...

Classical swine fever (CSF) is a highly contagious viral disease of pigs that has a tremendous socioeconomic impact. Vaccines are available for disease control. However, most industrialized countries are implementing stamping-out strategies to eliminate the disease and avoid trade restrictions. These restrictions can be avoided through the use of marker vaccines such as CP7_E2alf. Marker vaccin...

A new homogeneous immunoassay of whole blood C-reactive protein based on the use of magnetic fieldassisted agglutination of polyclonal anti-CRP coated superparamagnetic particles is described. These particles self-organize into linear chains under an appropriate magnetic field which considerably increase the colliding frequency and thus, the probability to form links between ligand and receptor...