This study investigated the natural incidence of deoxynivalenol (DON) in corn and products from corn-producing districts Punjab, Pakistan. The analysis was carried out using high performance liquid chromatography (HPLC) with UV detector immunoaffinity cleanup columns. detection limit (LOD) quantification were 25 50 µg/kg, respectively. A total 1220 samples analyzed to detect DON, 539 (44.2%) ob...

Attempts are frequently made to purify the desired proteins to the state of homogeneity by using an immunoaffinity column chromatography. The readymade immunoaffinity supports that are used in the process offer rapid and high-efficiency coupling of ligands, and are commercially available (1). Most of the laboratory work is spent testing the conditions that will ensure a highly efficient elution...

We have not had any problems using this method to purify proteins that are lethal on overexpression, including Abplp and Duolp. Although cells are dying during the induction, significant amounts of recombinant protein are produced. Because some proteins are sensitive to longer (>4 hr) induction times, it may be important to test protein expression at different time points during the induction. ...

Thromboxane synthase has been purified 620-fold from porcine lung microsomes by a three-step purification procedure including Lubrol-PX solubilization, reactive blue-agarose chromatography, and immunoaffinity chromatography. The purified enzyme exhibited a single protein band (53,000 daltons) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Rabbit antiserum raised against the purif...

Protein methylation is a common posttranslational modification that mostly occurs on arginine and lysine residues. Arginine methylation has been reported to regulate RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation is best known to regulate histone function and is involved in epigenetic regulation of gene transcription. To...

BACKGROUND Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable method for high-throughput clinical applications. Mass spectrometry (MS) can in ...

Replacement therapy for hemophilia B (factor IX deficiency) using prothrombin complex concentrate (PCC) has been associated with serious complications of thromboembolic events and transmission of viral infections. Monoclonal antibody-purified factor IX (Mononine) provides a highly purified factor IX concentrate, while eliminating other vitamin K-dependent factors (11, VII, and X). Mononine was ...