BACKGROUND The dust mites, which are mostly represented by Dermatophagoides spp. (Acari: Pyroglyphidae), are the major sources of indoor allergens. Identification and characterization of these mite allergen molecules are an important step in the development of new effective diagnostic procedures and possible therapeutic strategies for allergic disorders associated with dust mites. METHODS Tot...

Reversed-phase high performance liquid chromatographic (RPHPLC) determination of amino-acids with on-line pre-column ortho-phthalaldehyde (OPA) derivatization and fluorescence detection is rapid and sensitive. However, high-performance ionexchange chromatography (HP-IEC) with post-column ninhydrine reaction is the most widely used amino-acid (AA) assay for biological samples. These two methods ...

In cytopathic bovine viral diarrhea virus genotype 1 (BVDV1) isolates, insertions are reported at position A (amino acid [aa] 1535) and position B (aa 1589). Insertions at position B predominate. In this survey it was found that in BVDV2, insertions at position A predominate. Possible reasons for this difference in relative frequency are discussed.

The aim of this study was to investigate the amino acid (AA) release from breads enrichment with edible insects, Alphitobius diaperinus and Tenebrio molitor or pea protein during in vitro gastrointestinal digestion. Bread enriched at 5 10% insect flour protein. Enriched control were subjected standardised static free AAs before after each phase digestion (gastric, intestinal end digestion) dete...

Imiquimod (IMQ), a TLR7 agonist, is standard local treatment for non-melanoma skin cancers (NMSC). IMQ triggers inflammation, ultimately resulting in immunological tumor destruction. Albeit promotes the recruitment of effector T (TE) cells, anergy CD4+ cells. Meanwhile, how affects human CD8+ TE pivotal to cancer control, remains unclear. To address this, we studied cell lines from NMSC and cir...

We have determined the molecular basis for hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency in a patient, J.H., with Lesch-Nyhan syndrome. Radioimmunoassay of lysates of erythrocytes or cultured B-lymphoblasts showed that this patient had no detectable HPRT enzyme activity or HPRT protein. HPRT-specific mRNA levels were normal by Northern analysis. We created a cDNA library from...

BACKGROUND The early/asymptomatic stages of heart failure (HF) are characterized by sodium retention secondary to derangement of sodium reabsorption at the proximal nephron level. Because this phenomenon is reversed by ACE inhibition, abnormalities of renal sodium handling may depend on intrarenal changes of angiotensin II (AII)/nitric oxide (NO) levels. Renal hemodynamic reserve (ie, the glome...

Toll-interacting protein (Tollip) is a critical regulator of TOLL- like receptor (TLR)-signaling pathway. It predominantly associated with TLR2 and TLR4 during acute inflammatory conditions inhibits the TLR-mediated nuclear factor-kappa activation by suppressing autophosphorylation interleukin-1 receptor-associated kinase its activity. This article describes Tollip Labeo rohita (LrTollip), high...

A s adaptor molecules linking the codons in a mRNA to the amino acids that they specify, aminoacyl-tRNAs (AAtRNAs) play a central role in protein biosynthesis. In addition to this critical role, AA-tRNAs are also involved in several other less well known but still important biochemical reactions (Fig. 1). For example, AA-tRNAs are used as substrates for transfer of a single amino acid to the N ...

Reassortant SG3 inherits only the acidic polymerase (PA) protein gene from the cold-adapted B/AA/1/66 influenza virus (ca B/AA/1/66) and all remaining genes from a virulent, wild-type virus. This reassortant demonstrates attenuated virulence in ferrets and expresses a ts phenotype characteristic of the ca parent. During virulence evaluation of SG3, a virulent, non-ts revertant virus (designated...