نتایج جستجو برای: ihc

تعداد نتایج: 5931  

Journal: :Revista espanola de enfermedades digestivas : organo oficial de la Sociedad Espanola de Patologia Digestiva 2009
M L de Castro A Tardío V del Campo A Estévez J R Pineda F Domínguez J A Hermo J Clofent

BACKGROUND the role that cytomegalovirus (CMV) plays in inflammatory bowel disease (IBD) is controversial. The diagnosis of CMV infection in IBD depends on viral identification with hematoxylin-eosin (HE) or immunohistochemistry (IHC). Our aim was to compare the sensitivity of HE and IHC for this diagnosis in IBD patients. PATIENTS AND METHODS a case-control study. Our database was searched f...

2016
Shuo Wang Wanming Li Dezheng Yuan Jindan Song Jin Fang

The large external antigen (LEA) is a cell surface glycoprotein that has been proven to be highly expressed in colorectal cancer (CRC) as a tumor-associated antigen. To evaluate and validate the relationship between LEA expression and clinical characteristics of CRC with high efficiency, LEA expression levels were detected in 85 tissue blocks from CRC patients by quantum dot-based immunohistoch...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2008
Michael F Press Richard S Finn David Cameron Angelo Di Leo Charles E Geyer Ivonne E Villalobos Angela Santiago Roberta Guzman Armen Gasparyan Yanling Ma Kathy Danenberg Anne Marie Martin Lisa Williams Cristina Oliva Steven Stein Robert Gagnon Michael Arbushites Maria T Koehler

PURPOSE Biomarkers from two randomized phase III trials were analyzed to optimize selection of patients for lapatinib therapy. EXPERIMENTAL DESIGN In available breast cancer tissue from EGF30001 (paclitaxel +/- lapatinib in HER-2-negative/unknown metastatic breast cancer, n = 579) and EGF100151 (capecitabine +/- lapatinib in HER-2-positive metastatic breast cancer, n = 399), HER-2 gene amplif...

Journal: :Neuro-oncology 2013
Shadi Lalezari Arthur P Chou Anh Tran Orestes E Solis Negar Khanlou Weidong Chen Sichen Li Jose A Carrillo Reshmi Chowdhury Julia Selfridge Desiree E Sanchez Ryan W Wilson Mira Zurayk Jonathan Lalezari Jerry J Lou Laurel Ormiston Karen Ancheta Robert Hanna Paul Miller David Piccioni Benjamin M Ellingson Colin Buchanan Paul S Mischel Phioanh L Nghiemphu Richard Green He-Jing Wang Whitney B Pope Linda M Liau Robert M Elashoff Timothy F Cloughesy William H Yong Albert Lai

BACKGROUND Promoter methylation of the DNA repair gene, O-6-methylguanine-DNA methyltransferase (MGMT), is associated with improved treatment outcome for newly diagnosed glioblastoma (GBM) treated with standard chemoradiation. To determine the prognostic significance of MGMT protein expression as assessed by immunohistochemistry (IHC) and its relationship with methylation, we analyzed MGMT expr...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 1993
J M Miller A L Jenny W D Taylor R F Marsh R Rubenstein R E Race

Prion protein (PrP), which is involved in the pathogenesis of scrapie, occurs in 2 forms. The form extracted from scrapie brain is protease resistant (PrP-res), whereas PrP from normal brain is protease sensitive (PrP-sen). This study examined whether PrP-res could be detected in brains of sheep with scrapie by immunohistochemistry (IHC). A suitable IHC procedure was developed using brain tissu...

Journal: :Journal of clinical pathology 2008
C L Hyun H E Lee K S Kim S-W Kim J H Kim G Choe S Y Park

AIM To investigate the effect of polysomy 17 on HER-2 status as evaluated by immunohistochemistry (IHC), dual-colour fluorescence in situ hybridisation (FISH) and chromogenic in situ hybridisation (CISH). METHODS Dual-probe FISH and single-probe CISH were performed to detect HER-2 gene amplification, and IHC to detect HER-2 expression, on 309 invasive breast cancers. RESULTS Polysomy 17 was...

Journal: :Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2013
Brandie Heald Thomas Plesec Xiuli Liu Rish Pai Deepa Patil Jessica Moline Richard R Sharp Carol A Burke Matthew F Kalady James Church Charis Eng

PURPOSE In 2009, the Evaluation of Genomic Applications in Practice and Prevention recommended that all colorectal cancers (CRCs) be screened for Lynch syndrome (LS) through microsatellite instability (MSI) or immunohistochemistry (IHC). No studies report how this process is implemented on a health system-wide basis. METHODS Since 2004, Cleveland Clinic has screened CRC specimens with MSI/IHC...

Journal: :Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2016
Emma H Allott Stephanie M Cohen Joseph Geradts Xuezheng Sun Thaer Khoury Wiam Bshara Gary R Zirpoli C Ryan Miller Helena Hwang Leigh B Thorne Siobhan O'Connor Chiu-Kit Tse Mary B Bell Zhiyuan Hu Yan Li Erin L Kirk Traci N Bethea Charles M Perou Julie R Palmer Christine B Ambrosone Andrew F Olshan Melissa A Troester

BACKGROUND Classification of breast cancer into intrinsic subtypes has clinical and epidemiologic importance. To examine accuracy of IHC-based methods for identifying intrinsic subtypes, a three-biomarker IHC panel was compared with the clinical record and RNA-based intrinsic (PAM50) subtypes. METHODS Automated scoring of estrogen receptor (ER), progesterone receptor (PR), and HER2 was perfor...

2017
Chen Xu Yalan Liu Xiaowen Ge Dongxian Jiang Ying Zhang Yuan Ji Jun Hou Jie Huang Jieakesu Su Haiying Zeng Jing Qin Yingyong Hou

BACKGROUND HER2 assessment in biopsy specimens of gastric cancer (GC) is challenging because of the intratumoral heterogeneity. False negative results may be get because of limited biopsy material. The aim of this study is to explore how tumor-containing fragment number and biopsy specimen number affect HER2 immunohistochemistry (IHC) positive rate. METHODS Eight hundred and ninety biopsy spe...

2012
Young Kyung Bae Gyungyub Gong Jun Kang Ahwon Lee Eun Yoon Cho Ji Shin Lee Kwang-Sun Suh Dong Wha Lee Woo Hee Jung

PURPOSE Amplification of the human epidermal growth factor receptor 2 (HER2) gene occurs in 18% to 20% of breast cancers, and it is recognized as a prognostic and predictive marker. We investigated the HER2 status in Korean breast cancer by immunohistochemistry (IHC) and silver-enhanced in situ hybridization (SISH), as the first step toward building a nationwide quality assurance program for HE...

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