Transforming growth factor beta (TGFbeta) is known to exert both positive and negative effects on different stages of tumor formation. Of the TGFbetaisoforms, TGFbeta1 is highly expressed in prostate cancer and leads to tumor promotion and metastasis. Increased expression of TGFbeta1 is associated with more aggressive tumors and poor prognosis. Several polymorphisms in TGFB1 have been identifie...

Objective. To determine active TGF-β1 (aTGF-β1) levels in serum, skin, and peripheral blood mononuclear cell (PBMC) culture supernatants and to understand their associations with clinical parameters in systemic sclerosis (SSc) patients. Methods. We evaluated serum samples from 56 SSc patients and 24 healthy controls (HC). In 20 SSc patients, we quantified spontaneous or anti-CD3/CD28 stimulated...

Studies using genetic isolates with limited genetic variation may be useful in chronic obstructive pulmonary disease (COPD) genetics, but are thus far lacking. The associations between single nucleotide polymorphisms (SNPs) in candidate genes and lung function in COPD were studied in a genetic isolate. In 91 subjects with Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage >or=1...

Vitiligo is an autoimmune skin disease characterized by depigmentation, caused autoreactive IFNg-producing CD8+ T cells (CD8s) that target melanocytes and promote their destruction. Using single-cell RNA sequencing from the of vitiligo patients as well healthy controls, we determined CD8s directly communicate through TGFb signaling, with CD8-specific production TGFb1 melanocyte-specific TGFb2. ...

Ionizing radiation causes DNA damage that elicits a cellular program of damage control coordinated by the kinase activity of ataxia telangiectasia mutated protein (ATM). Transforming growth factor B (TGFB)-1, which is activated by radiation, is a potent and pleiotropic mediator of physiologic and pathologic processes. Here we show that TGFB inhibition impedes the canonical cellular DNA damage s...

TGF-β plays a critical role in normal mammary development and morphogenesis. Decreased TGF-β signaling has been associated with increased mammographic density. Percent mammographic density (PMD) adjusted for age and body mass index (BMI) is a strong risk factor and predictor of breast cancer risk. PMD is highly heritable, but few genetic determinants have been identified. We investigated the as...

صخلملا Infertility affects 15% of couples seeking children worldwide and male factor is evident in about 30-50% of the cases. To assess the effect of sperm chromatin integrity on the outcomes of intracytoplasmic injection, sperm DNA compactness was evaluated by acridine orange staining in 40 fertile and 44 infertile men attending one infertility clinic. The results showed no significant differe...

صخلملا The search for novel anticancer drugs continues. This work includes a preliminary study of the effect of two crude extracts of Greek or Mediterranean sage (Salvia triloba L. f.) on three malignant human cell lines and one malignant murine cell line. A boiling water extract and a methanolic extract were prepared from dried leaves of S. triloba. Yields of extraction were 9.8 and 22.4%, res...

BACKGROUND The mechanical stress that the human diaphragm is exposed to during mechanical ventilation affects a variety of processes, including signal transduction, gene expression, and angiogenesis. OBJECTIVES The study aim was to assess the change in the production of major angiogenic regulators [vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF2), and transforming ...

Osteosarcoma (OS) is the most common histological type of primary bone cancer. The present study was designed to identify the key genes and signaling pathways involved in the metastasis of OS. Microarray data of GSE39055 were downloaded from the Gene Expression Omnibus database, which included 19 OS biopsy specimens before metastasis (control group) and 18 OS biopsy specimens after metastasis (...