نتایج جستجو برای: hygro
تعداد نتایج: 245 فیلتر نتایج به سال:
In this article, the problem of time-dependent stress redistribution of a piezomagnetic rotating thick-walled cylinder under an axisymmetric hygro-thermo-magneto-electro-mechanical loading is analyzed analytically for the condition of plane strain. Using the constitutive equations, a differential equation is found in which there are creep strains. Primarily, eliminating creep strains, an analyt...
Embryonic stem (ES) cells display the ability to differentiate in vitro into a variety of cell lineages. Using a cell-trapping system, we have obtained an insulin-secreting cell clone from undifferentiated ES cells. The construction used allows the expression of a neomycin selection system under the control of the regulatory regions of the human insulin gene. The chimeric gene also contained a ...
The genes for Nipah virus (NiV) proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed, and purified using immobilized metal affinity chromatography. The recombinant N, F, and G NiV proteins (rNiV-N, rNiV-F, and rNiV-G), were successfully expressed in Escherichia coli and purified with a yield of 4, 16, and 4 mg/L, respectively. All 3 recombinant viral protein...
Abstrad C127 is a nontumorigenic mouse cell line widely used in in vitro transformation assays due to its normal morphological appearance and its very low levels of spontaneous transformation. We now report that C127 cells are resistant to transformation by tyrosine protein kinase oncogenes derived from growth fador receptors such as the retroviral v-fms and the human trk transforming genes. In...
Introduction Conclusions References Tables Figures Back Close Full Screen / Esc Abstract Introduction Conclusions References Tables Figures Back Close Full Screen / Esc Printer-friendly Version Interactive Discussion EGU Abstract We present a method to describe the relationship between dry particle diameter and cloud condensation nuclei (CCN) activity using a single hygroscopicity parameter. Va...
Within the large group of Estrogen Receptor alpha (ERα)-negative breast cancer patients, there is a subgroup carrying the phenotype ERα(-), PR(-), and Her2(-), named accordingly "Triple-Negative" (TN). Using cell lines derived from this TN group, we wished to establish cell clones, in which ERα is ectopically expressed, forming part of a synthetic lethality screening system. Initially, we gener...
We have shown previously that a mutation of the KI-KII site immediately 5' to J(kappa)1 on the mouse immunoglobulin light chain kappa locus reduces the rearrangement level in cis, although it does not affect transcription. Here we deleted by homologous recombination in mouse embryonic stem cells a 4-kb DNA fragment, located immediately upstream of the KI-KII element, which contains the promoter...
During the service life of buildings, natural aging and eventual damage of materials due to different chemical, physical, and biological processes can take place. Ageing of the materials is one aspect of the environmental processes and involve different chemical, mechanical and biological reactions of the materials. Bio-deterioration, e.g. mould, decay and insect damage in buildings, is caused ...
We document the hygroscopic swelling and shrinkage of the central and the thickest secondary cell wall layer of wood (named S2) in response to changes in environmental humidity using synchrotron radiation-based phase contrast X-ray tomographic nanoscopy. The S2 layer is a natural fibre-reinforced nano-composite polymer and is strongly reactive to water. Using focused ion beam, micropillars with...
مقدمه: ساب کلونینگ ژنهای اختصاصی در پلاسمیدها و انتقال آنها به سلولهای هدف به منظور تولید پروتئینهای درمانی یا تمایز سلول به عنوان یکی از مؤثرترین روشهای درمان برای بیماریهای مختلف پیشنهاد میشود. فاکتور رشد عصب (NGF) یکی از اعضای خانواده نوروتروفینها است. نوروتروفینها خانوادهای از پروتئینها هستند که میزان بقاء، تمایز و عملکرد انواع مختلف نورونها را تنظیم میکنند. مطالعات نشان دادندک...
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