Rosin natural terpene (Dertoline) is compounded with a bacterial thermoplastic polyesters polyhydroxyalkanoate (PHA), specifically here poly(3-hydroxybutyrate) (PHB), biodegradable and biocompatible polymer. The compatibility of the blend examined differential scanning calorimetry thermal gravimetry analysis. In range concentration Dertoline, from 5 to 15 wt%, cold crystallization peak first sh...

Development of experimental models of proteins sustained release is a promising trend in modern biochemistry and biopharmacology. Application of proteins sustained release systems based on biopolymer microparticles allow to model prolonged protein action in vitro and in vivo. Moreover, the use of these devices in biopharmacology can eliminate the most of the drawbacks of traditional medicines: ...

BACKGROUND In recent years the production of biobased biodegradable plastics has been of interest of researchers partly due to the accumulation of non-biodegradable plastics in the environment and to the opportunity for new applications. Commonly investigated are the polyhydroxyalkanoates (PHAs) poly(hydroxybutyrate) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHB-V). The latter has the advan...

The acetoacetyl-CoA reductase and the polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha (formerly Alcaligenes eutrophus) were expressed in Escherichia coli, Klebsiella aerogenes, and PHA-negative mutants of R. eutropha and Pseudomonas putida. While expression in E. coli strains resulted in the accumulation of poly(3-hydroxybutyrate) [PHB], strains of R. eutropha, P. putida and K. aero...

Oxygen supply implies higher production cost and reduction of maximum theoretical yields. Thus, generation fermentation products is more cost-effective. Aiming to find a key piece for the (poly)-3-hydroxybutyrate (PHB) as product, here we characterize an acetoacetyl-CoA reductase, isolated from Candidatus Accumulibacter phosphatis-enriched mixed culture, showing (kcatNADH/KMNADH)/(kcatNADPH/KMN...

Waste paper holds great potential as a substrate for the microbial production of bioplastic (Poly-3-hydroxybutyrate (PHB)). This study aimed to produce PHB by utilizing office using Cupriavidus necator through batch and fed-batch simultaneous saccharification fermentation (SSF) approach. For experiment, different loadings shredded (3, 5 10%) with two pretreatments H2O2 (OPH) Triton X-100 (OPTH)...

Like many other prokaryotes, rhizobacteria of the genus Azospirillum produce high levels of poly(beta-hydroxybutyrate) (PHB) under suboptimal growth conditions. Utilization of PHB by bacteria under stress has been proposed as a mechanism that favors their compatible establishment in competitive environments, thus showing great potential for the improvement of bacterial inoculants for plants and...

Like many other prokaryotes, rhizobacteria of the genus Azospirillum produce high levels of poly-beta-hydroxybutyrate (PHB) under sub-optimal growth conditions. Utilization of PHB by bacteria under stress has been proposed as a mechanism that favors their compatible establishment in competitive environments. PHB depolymerase (PhaZ) is an essential enzyme in PHB degradation. The phaZ gene was id...