The purpose of this report is to record several observations made in a study of hydronephrosis produced in rats by unilateral ligation of the ureter. I t has been known from the work of Wilmer (1) and Wachstein (2, 3) that the staining of the proximal tubule brush border for alkaline phosphatase activity is diminished in experimental hydronephrosis. However, the speed with which this decrease o...