Water Gas Shift Reaction: The turn-over-rate (TOR) for the water gas shift (WGS) reaction of 1.4 nm Au/Al2O3 is approximately 20 times higher than that of 1.6 nm Pt/Al2O3. In addition, Au has high activity at lower reaction temperature than Pt. Operando XAFS experiments at both the Au and Pt L3 edges reveal that under reaction conditions, the catalysts are fully metallic. Adsorption of CO, H2 a...

Background and Study Aim: Recently, immunohistochemistry has shown numerous IgG4-positive plasma cells in a subset of Hashimoto’s thyroiditis (HT), and this type of HT (IgG4 HT) appears to be a subtype of IgG4-related sclerosing disease. However, little is known about the distribution pattern of plasma cells in IgG4 thyroiditis. To clarify the distribution pattern of IgG4-positive plasma cells,...

Background and Study Aim: Recently, immunohistochemistry has shown numerous IgG4-positive plasma cells in a subset of Hashimoto’s thyroiditis (HT), and this type of HT (IgG4 HT) appears to be a subtype of IgG4-related sclerosing disease. However, little is known about the distribution pattern of plasma cells in IgG4 thyroiditis. To clarify the distribution pattern of IgG4-positive plasma cells,...

Differentiating immunohistochemically stained bacteria on tissue sections, that possess high levels of melanomacrophages and free melanin can be difficult. This can be compounded due to the routine use of the chromogen diaminobenzidine for immunohistochemical procedures. Here we describe a technique for melanin bleaching prior to immunostaining to remove the subjectivity from results when study...

Vajner L. , V. Konrádová, J . Uhl ík , J . Zocová: Mucin Histochemistry of Tracheal Goblet Cells after Oral Administration of Ambroxol. Acta Vet. Brno 2001, 70: 9-13. Previous studies on the effect of various mucolytic drugs on the tracheal epithelium ultrastructure revealed ambroxol as the most harmful one. To complete these studies, we decided to evaluate the effect of ambroxol on the glycoco...

Vajner L. , J . Uhl ík , V. Konrádová, J . Zocová: The Effect of Intravenously Administered Acetylcholine on the Glycoconjugate Composition in Goblet Cells of the Tracheal Epithelium in Rabbits. Acta Vet. Brno 2000, 69: 17-23. To evaluate the influence of cholinergic stimulation on the composition of glycoconjugates in goblet cells, we studied the tracheal epithelium of rabbits 5 minutes and 20...

Purpose: Cytosolic phospholipase A2-a (cPLA2-a) provides intracellular arachidonic acid to supply both cyclooxygenase and lipoxygenase pathways.We aim to determine the expression and activation of cPLA2-a in prostate cancer cell lines and tissue and the effect of targeting cPLA2-a in vitro and in vivo. Experimental Design:The expression of cPLA2-a was determined in prostate cancer cells by reve...

The pharmacodynamics of doxorubicin in human prostate tumors were studied using histocultures of radical prostatectomy specimens. Drug treatment lasted 96 h. The antiproliferative effect was measured by the inhibition of DNA precursor ([3H]thymidine) incorporation, and the cytotoxic effect was measured by monitoring cells with fragmented DNA, as indicated by the terminal deoxynucleotidyl transf...

Long noncoding RNAs play critical roles in cellular homeostasis, and long RNA H19 (H19) is implicated several pathologic conditions. The putative role of the pathogenesis progression hypoxic-ischemic brain damage (HIBD) not yet understood. Therefore, a series vivo vitro experiments were designed to investigate potential neuronal apoptosis cognitive dysfunction HIBD. expression was decreased HIB...

Purpose:There is an urgent need for new therapeutic strategies in Ewing’s sarcoma family of tumors (ESFT). In this study, we have evaluated the effect of fenretinide [N-(4-hydroxyphenyl)retinamide] in ESFT models. Experimental Design:The effect of fenretinide on viable cell number and apoptosis of ESFT cell lines and spheroids and growth of s.c. ESFT in nu/nu mice was investigated. The role of ...