This present paper deals with the development and validation of a stability indicating high performance liquid chromatographic method for the quantitative determination of Memantine hydrochloride. Memantine hydrochloride was derivatized with 0.015 M 9-fluorenylmethyl chloroformate (FMOC) and 0.5 M borate buffer solution by keeping it at room temperature for about 20 minutes and the chromatograp...

Several antagonistic interactions between morphine and LSD-25 (lysergic acid diethylamide) have been demonstrated. LSD-25 antagonizes morphine induced analgesia in rats (1) and emesis in dogs (2). It also blocks the miotic action of morphine (3). On the other hand morphine blocks LSD-25 induced pyre xia in rabbits (4) and the central hypotensive effect of LSD-25 in dogs (5). Morphine produces m...

A strain of Klebsiella aerogenes is described which was unable to grow from light inocula on 5% lysed horse blood diagnostic sensitivity test (DST) agar. It was able to grow on DST agar without added horse cells and MacConkey and nutrient agar. Growth on a deficient medium was promoted by sulphonamides and nitrofurantoin as well as by para-amino benzoic acid, para-amino salicylic acid, procaine...

Potassium permanganate (KMnO4) and oleic acid (OA) were purchased from Alfa Aesar. Glutathione (GSH), 3-(4,5-dimethylthialzol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alpha lipoic acid (ALA), buthionine sulfoximine (BS) and N-ethylmaleimide (NEM) were purchased from Sigma-Aldrich. Doxorubicin hydrochloride (DOX) was purchased from Dingguo reagent company (Beijing, China). All buffers were p...

Using quality by design approaches (dispersion analysis and random balance method), the formulation technology of powder for oral solution in sachets were developed. It includes paracetamol, ascorbic acid, phenylephrine hydrochloride, pheniramine maleate. The influence 27 excipients from 5 functional groups on 11 attributes was established dispersion analysis. preferred selected using utility f...

The use of benzidine hydrochloride to precipitate sulfates as insoluble benzidine sulfate was first proposed by Miiller (1902) for the determination of free and bound sulfur in minerals. Subsequently Rosenheim and Drummond (1914) and Fiske (1921) used it for the determination of sulfates in urine. Later Fiske (1922) described a method for the determination of total base in urine, the bases afte...

A novel approach was used to develop and validate a rapid, specific, accurate and precise reverse phase ultra performance liquid chromatographic (UPLC) method for the simultaneous determination of Sitagliptin phosphate monohydrate and Metformin hydrochloride in pharmaceutical dosage forms. The chromatographic separation was achieved on Aquity UPLC BEH C8 100 × 2.1 mm, 1.7 μm, column using a buf...

Hydrodynamic, chemical, and optical properties of arginine deiminase (EC 3.5.3.6) from Mycoplasma arthritidis are reported for the enzyme isolated from log phase cells. The s& +, and D’& values of the enzyme are 5.48 S and 5.87 x 10V7 cm’/s, respectively; the molecular weight is 87,300. Determination of the amino acid composition shows that about 45% of the residues are nonpolar. Another unique...

The effect of NMDA (N-methyl-D-aspartate) receptor antagonists on tolerance to morphine antinociception was investigated in mice. Daily subcutaneous administration of 50 mg/kg of morphine hydrochloride for three days induced tolerance to different (3,6 and 9 mg/kg) test doses of morphine. The tolerance obtained was decreased by pretreatment of animals with single or repeated doses of compe...

the effect of nmda (n-methyl-d-aspartate) receptor antagonists on tolerance to morphine antinociception was investigated in mice. daily subcutaneous administration of 50 mg/kg of morphine hydrochloride for three days induced tolerance to different (3,6 and 9 mg/kg) test doses of morphine. the tolerance obtained was decreased by pretreatment of animals with single or repeated doses of competitiv...