Introduction The two-hybrid system (THS), first introduced by Fields and Song (1989) (Ref. 1), is a powerful technique for identifying new proteins involved in specific biological processes. It allows for the rapid isolation of the gene that codes for a protein that interacts with a specific protein of interest. Here, we present not a review of the current THS technology but, rather, a comprehe...

The development and implementation of a novel structural testing method involving the combined use of shake tables, actuators, and computational engines for the seismic simulation of structures is presented herein. The hybrid simulation is intended to discover through physical testing the behavior of parts or whole substructure assemblies for which knowledge is limited, while the known parts of...

in order to obtain high yielded and tolerant to drought stress maize hybrid a breeding program was started in safiabad agricultural research center in the 1999 and a few s6 lines attained in 2005. then interactive components were created and evaluated in the preliminary and semi-finals experiment during two consecutive years. after preliminary studies, nine hybrids were selected which they were...

in this paper, optimal design of hybrid solar-wind- diesel energy system with battery storage for reliable load in arsoon village in ardabil is presented. the village has 35 households that mean daily energy consumption is estimated 63.4 kwh. in this design optimization is implemented, considering loss of power supply probability (lpsp) index for reliable load providing, with minimum total cons...

In hybrid simulation, the structure is divided into numerical and physical substructures to achieve more accurate responses in comparison to a full computational analysis. As a consequence of the lack of test facilities and actuators, and the budget limitation, only a few substructures can be modeled experimentally, whereas the others have to be modeled numerically. In this paper, a new hybrid ...

RESEARCH ARTICLE Expression and Construction of Yeast Expression Vector Containing CsTCTP1 Gene from Cucumber in Yeast Two-Hybrid System Qiu-Min Chen, Na Cui , Yang Yu, Xiang-Nan Meng and Hai-Yan Fan 2,* College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural Uni...

Many authors have examined lot sizing, scheduling and sequence of multi-product flow shops, but most of them have assumed that set up times are independent of sequence. Whereas dependence of set up times to sequence is more common in practice. Hence, in this paper, we examine the discussed problem with hypothesis of dependence of set up times to sequence and cyclic schedule policy in basic peri...

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

The tensile properties of multiscale, hybrid, thermoplastic-based nanocomposites reinforced with nano-CaCO3 particles and micro–short glass fibers (SGF) were predicted by a two-step, three-dimensionalmodel using ANSYS finite element (FE) software. Cylindrical and cuboid representative volume elements were generated to obtain the effective behavior of the multiscale hybrid composites. In the fir...