D URING the last ten years evidence has been presented from different laboratories that fibrinogen may not be essential for thrombin-mediated platelet aggregation.14 Although the presence, in platelets, of a thrombinsensitive protein other than fibrinogen has been reported from this laboratory and also by other workers,5 #{176} there is no report of its isolation, structure, physicochemical pro...

Plasminogen (profibrinolysin) of high specific activity has been prepared from human sources and converted to plasmin (fibrinolysin) by a variety of means (3, 4). Since these preparations seemed to possess activities of as high an order as any that had yet been produced, it was considered appropriate to examine their purity by physicochemical means. Such investigations do not appear to have bee...

Gangliosides, glycosphingolipids which contain sialic acid, were studied in human platelets. They represented 0.5% of the platelet lipids and accounted for 6% of the total neuraminic acid content of platelets. Three major ganglioside fractions were identified and characterized. Ganglioside I was hematoside (G(6)) and comprised 92% of the platelet gangliosides. It contained glucose, galactose, a...

L YMPHOID TISSUES of mice injected with Rauscher leukemia virus have been shown, by immunofluorescent technics, to contain antigen ( s ) reactive with antimurine leukemia antibody prepared against the Rauscher strain.’ Other immunofluorescent studies2’3 have demonstrated that blood and bone marrow cells from patients with leukemia, lymphoma, Hodgkin’s disease, polycythemia, teratoma, mycosis fu...

1. Comparison of peptide maps of the Fc fragments of normal G immunoglobulins and 11 G myeloma proteins of the We (b) type showed them to be very similar except for differences associated with the Gm type. Some additional differences were noted, however, in the Fc fragments of three Vi (c) myeloma proteins. 2. Peptide maps of heavy chains from the same G myeloma proteins differed from each othe...

Let me tell you at the outset of my appreciation of the great honour you have done me in asking me to deliver the first Lawson Wilkins Memorial Lecture. I accepted this invitation with eagerness because I cherish any link that binds Lawson Wilkins' life and work to mine. I often turn to the page in the second edition of his great textbook in which he proudly listed the 31 Associates and Fellows...

Dichloroacetate (DCA), a stimulator of the pyruvate dehydrogenase complex, decreases lactate levels and peripheral resistance and increases cardiac output. This study was performed to examine the effects of DCA on exercise performance in humans. Eight healthy male volunteers (age 20–28 years) were tested by bicycle spiro-ergometry using a microprocessor-controlled gas analysis system after infu...

The Donath-Landsteiner reaction was studied using low and high titer antisera and purified antibody, normal and PNH erythrocytes, and human serum complement. The requirement for complement in both the cold and warm phases of the reaction depended upon the level of antibody used and the sensitivity of the cells to hemolytic antibodies. Complement was not necessary in the cold phase using PNH cel...

High-speed (80,000 X g) pellets of plasma from leukemic patients and from normal individuals were used to prepare antisera in rabbits. The leukemic plasma pellets contained virus-like particles as determined by electron microscopy, whereas similar particles were not observed in the normal pellets. Globulin fractions from six antisera to normal pellets (NPA) and six antisera to leukemic pellets ...